Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity

J. A. Ogden, J. A. Tate, R. R. Strawbridge, Robert Ivkov, P. J. Hoopes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of medical grade iron oxide nanoparticles (IONP) has renewed interest in hyperthermia cancer therapy. Because of their modifiable size and heating capabilities under an AC magnetic field (alternating magnetic field, AMF), IONPs have the potential to damage or kill cells in a manner more therapeutically efficient than previous hyperthermia techniques. The use of IONPs in hyperthermia cancer therapy has prompted numerous questions regarding the cytotoxic mechanism associated with IONP heat therapy and if such mechanism is different (more or less effective) with respect to conventional hyperthermia techniques. In this in vitro study, we determine the immediate and long-term (24 hours) cytotoxic effects of isothermal IONP hyperthermia treatment versus a conventional global heating technique (water bath). Using the same heating time and temperature we showed significantly greater cytotoxicity in IONP-heated cells as opposed to water bath-treated cells. We postulate that the difference in treatment efficacy is due to the spatial relationship of particle-induced thermal damage within cells. Although the exact mechanism is still unclear, it appears likely that intracellular IONPs have to achieve a very high temperature in order to heat the surrounding environment; therefore it is reasonable to assume that particles localized to specific areas of the cell such as the membrane can deliver exacerbated injury to those areas. In this experiment, although detectable global temperature for the particle-heated cells stands comparable to the conventional heat treatment, particle-induced cell death is higher. From the results of this study, we propose that the mechanism of IONP hyperthermia renders enhanced cytotoxicity compared to conventional waterbath hyperthermia at the same measured thermal dose.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7181
DOIs
StatePublished - 2009
Externally publishedYes
EventEnergy-based Treatment of Tissue and Assessment V - San Jose, CA, United States
Duration: Jan 25 2009Jan 26 2009

Other

OtherEnergy-based Treatment of Tissue and Assessment V
CountryUnited States
CitySan Jose, CA
Period1/25/091/26/09

Fingerprint

Hyperthermia
Cytotoxicity
hyperthermia
Iron oxides
iron oxides
Iron
Nanoparticles
Oxides
nanoparticles
Cell
cells
Heating
Therapy
therapy
Magnetic fields
heating
baths
Cancer
Water
Damage

Keywords

  • Alternating magnetic field
  • AMF
  • Efficacy
  • Hyperthermia
  • Iron oxide
  • MCF-7
  • Nanoparticle
  • TEM
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Ogden, J. A., Tate, J. A., Strawbridge, R. R., Ivkov, R., & Hoopes, P. J. (2009). Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7181). [71810K] https://doi.org/10.1117/12.809818

Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity. / Ogden, J. A.; Tate, J. A.; Strawbridge, R. R.; Ivkov, Robert; Hoopes, P. J.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7181 2009. 71810K.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogden, JA, Tate, JA, Strawbridge, RR, Ivkov, R & Hoopes, PJ 2009, Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7181, 71810K, Energy-based Treatment of Tissue and Assessment V, San Jose, CA, United States, 1/25/09. https://doi.org/10.1117/12.809818
Ogden JA, Tate JA, Strawbridge RR, Ivkov R, Hoopes PJ. Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7181. 2009. 71810K https://doi.org/10.1117/12.809818
Ogden, J. A. ; Tate, J. A. ; Strawbridge, R. R. ; Ivkov, Robert ; Hoopes, P. J. / Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7181 2009.
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