Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture

J. T. McClintock, S. R. Thaker, M. Mosher, D. Jones, M. Forman, P. Charache, K. Wright, J. Keiser, F. E. Taub

Research output: Contribution to journalArticle

Abstract

The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase - to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.

Original languageEnglish (US)
Pages (from-to)1554-1559
Number of pages6
JournalJournal of Clinical Microbiology
Volume27
Issue number7
StatePublished - 1989
Externally publishedYes

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DNA Probes
Cytomegalovirus
In Situ Hybridization
Cell Culture Techniques
Monoclonal Antibodies
Staining and Labeling
Horseradish Peroxidase
Routine Diagnostic Tests

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

McClintock, J. T., Thaker, S. R., Mosher, M., Jones, D., Forman, M., Charache, P., ... Taub, F. E. (1989). Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture. Journal of Clinical Microbiology, 27(7), 1554-1559.

Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture. / McClintock, J. T.; Thaker, S. R.; Mosher, M.; Jones, D.; Forman, M.; Charache, P.; Wright, K.; Keiser, J.; Taub, F. E.

In: Journal of Clinical Microbiology, Vol. 27, No. 7, 1989, p. 1554-1559.

Research output: Contribution to journalArticle

McClintock, JT, Thaker, SR, Mosher, M, Jones, D, Forman, M, Charache, P, Wright, K, Keiser, J & Taub, FE 1989, 'Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture', Journal of Clinical Microbiology, vol. 27, no. 7, pp. 1554-1559.
McClintock, J. T. ; Thaker, S. R. ; Mosher, M. ; Jones, D. ; Forman, M. ; Charache, P. ; Wright, K. ; Keiser, J. ; Taub, F. E. / Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture. In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 7. pp. 1554-1559.
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