Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray

Dasheng Zheng, Jun Wan, Yong Gu Cho, Leyao Wang, Chuang Jiun Chiou, Shweta Pai, Crystal Woodard, Jian Zhu, Gangling Liao, Otoniel Martinez-Maza, Jiang Qian, Heng Zhu, Gary Selwyn Hayward, Richard F Ambinder, S. Diane Hayward

Research output: Contribution to journalArticle

Abstract

Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.

Original languageEnglish (US)
Pages (from-to)1683-1691
Number of pages9
JournalJournal of Infectious Diseases
Volume204
Issue number11
DOIs
StatePublished - Dec 1 2011

Fingerprint

Human Herpesvirus 8
Proteome
Humoral Immunity
Human Herpesvirus 4
Protein Array Analysis
HIV-1
Immunoglobulin G
AIDS-Related Lymphoma
Viruses
Antigens
Viral Antigens
Herpesviridae
Viral DNA
Seroepidemiologic Studies
B-Cell Lymphoma
Viral Load
Immunoglobulin A
Antibody Formation
Healthy Volunteers
Proteins

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray. / Zheng, Dasheng; Wan, Jun; Cho, Yong Gu; Wang, Leyao; Chiou, Chuang Jiun; Pai, Shweta; Woodard, Crystal; Zhu, Jian; Liao, Gangling; Martinez-Maza, Otoniel; Qian, Jiang; Zhu, Heng; Hayward, Gary Selwyn; Ambinder, Richard F; Hayward, S. Diane.

In: Journal of Infectious Diseases, Vol. 204, No. 11, 01.12.2011, p. 1683-1691.

Research output: Contribution to journalArticle

Zheng, Dasheng ; Wan, Jun ; Cho, Yong Gu ; Wang, Leyao ; Chiou, Chuang Jiun ; Pai, Shweta ; Woodard, Crystal ; Zhu, Jian ; Liao, Gangling ; Martinez-Maza, Otoniel ; Qian, Jiang ; Zhu, Heng ; Hayward, Gary Selwyn ; Ambinder, Richard F ; Hayward, S. Diane. / Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray. In: Journal of Infectious Diseases. 2011 ; Vol. 204, No. 11. pp. 1683-1691.
@article{c4a5697b01344792a2d493d98e95acec,
title = "Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray",
abstract = "Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.",
author = "Dasheng Zheng and Jun Wan and Cho, {Yong Gu} and Leyao Wang and Chiou, {Chuang Jiun} and Shweta Pai and Crystal Woodard and Jian Zhu and Gangling Liao and Otoniel Martinez-Maza and Jiang Qian and Heng Zhu and Hayward, {Gary Selwyn} and Ambinder, {Richard F} and Hayward, {S. Diane}",
year = "2011",
month = "12",
day = "1",
doi = "10.1093/infdis/jir645",
language = "English (US)",
volume = "204",
pages = "1683--1691",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray

AU - Zheng, Dasheng

AU - Wan, Jun

AU - Cho, Yong Gu

AU - Wang, Leyao

AU - Chiou, Chuang Jiun

AU - Pai, Shweta

AU - Woodard, Crystal

AU - Zhu, Jian

AU - Liao, Gangling

AU - Martinez-Maza, Otoniel

AU - Qian, Jiang

AU - Zhu, Heng

AU - Hayward, Gary Selwyn

AU - Ambinder, Richard F

AU - Hayward, S. Diane

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.

AB - Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.

UR - http://www.scopus.com/inward/record.url?scp=80155198393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80155198393&partnerID=8YFLogxK

U2 - 10.1093/infdis/jir645

DO - 10.1093/infdis/jir645

M3 - Article

C2 - 21990424

AN - SCOPUS:80155198393

VL - 204

SP - 1683

EP - 1691

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 11

ER -