Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray

Dasheng Zheng; Jun Wan; Yong Gu Cho; Leyao Wang; Chuang Jiun Chiou; Shweta Pai; Crystal Woodard; Jian Zhu; Gangling Liao; Otoniel Martinez-Maza; Jiang Qian; Heng Zhu; Gary S. Hayward; Richard F. Ambinder; S. Diane Hayward

doi:10.1093/infdis/jir645

Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray

Dasheng Zheng, Jun Wan, Yong Gu Cho, Leyao Wang, Chuang Jiun Chiou, Shweta Pai, Crystal Woodard, Jian Zhu, Gangling Liao, Otoniel Martinez-Maza, Jiang Qian, Heng Zhu, Gary S. Hayward, Richard F. Ambinder, S. Diane Hayward

School of Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.

Original language	English (US)
Pages (from-to)	1683-1691
Number of pages	9
Journal	Journal of Infectious Diseases
Volume	204
Issue number	11
DOIs	https://doi.org/10.1093/infdis/jir645
State	Published - Dec 1 2011

ASJC Scopus subject areas

General Medicine

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Zheng, D., Wan, J., Cho, Y. G., Wang, L., Chiou, C. J., Pai, S., Woodard, C., Zhu, J., Liao, G., Martinez-Maza, O., Qian, J., Zhu, H., Hayward, G. S., Ambinder, R. F., & Hayward, S. D. (2011). Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray. Journal of Infectious Diseases, 204(11), 1683-1691. https://doi.org/10.1093/infdis/jir645

Zheng, D, Wan, J, Cho, YG, Wang, L, Chiou, CJ, Pai, S, Woodard, C, Zhu, J, Liao, G, Martinez-Maza, O, Qian, J , Zhu, H, Hayward, GS, Ambinder, RF & Hayward, SD 2011, 'Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray', Journal of Infectious Diseases, vol. 204, no. 11, pp. 1683-1691. https://doi.org/10.1093/infdis/jir645

@article{c4a5697b01344792a2d493d98e95acec,

title = "Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray",

abstract = "Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.",

author = "Dasheng Zheng and Jun Wan and Cho, {Yong Gu} and Leyao Wang and Chiou, {Chuang Jiun} and Shweta Pai and Crystal Woodard and Jian Zhu and Gangling Liao and Otoniel Martinez-Maza and Jiang Qian and Heng Zhu and Hayward, {Gary S.} and Ambinder, {Richard F.} and Hayward, {S. Diane}",

note = "Funding Information: Financial support. This work was supported by Public Health Service grants from the National Cancer Institute of the National Institutes of Health (R37 CA42245 and R01 CA30356 to S. D. H, R21 CA138163 to S. D. H. and H. Z., RC2 CA148402 to G. S. H., and Cancer Center Core Grant P30CA006973 to William Nelson). Potential conflicts of interest. All authors: No reported conflicts.",

year = "2011",

month = dec,

day = "1",

doi = "10.1093/infdis/jir645",

language = "English (US)",

volume = "204",

pages = "1683--1691",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

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TY - JOUR

T1 - Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray

AU - Zheng, Dasheng

AU - Wan, Jun

AU - Cho, Yong Gu

AU - Wang, Leyao

AU - Chiou, Chuang Jiun

AU - Pai, Shweta

AU - Woodard, Crystal

AU - Zhu, Jian

AU - Liao, Gangling

AU - Martinez-Maza, Otoniel

AU - Qian, Jiang

AU - Zhu, Heng

AU - Hayward, Gary S.

AU - Ambinder, Richard F.

AU - Hayward, S. Diane

N1 - Funding Information: Financial support. This work was supported by Public Health Service grants from the National Cancer Institute of the National Institutes of Health (R37 CA42245 and R01 CA30356 to S. D. H, R21 CA138163 to S. D. H. and H. Z., RC2 CA148402 to G. S. H., and Cancer Center Core Grant P30CA006973 to William Nelson). Potential conflicts of interest. All authors: No reported conflicts.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.

AB - Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.

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SN - 0022-1899

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SP - 1683

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JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 11

ER -

Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray

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