TY - JOUR
T1 - Comparison of freshly isolated adipose tissue-derived stromal vascular fraction and bone marrow cells in a posterolateral lumbar spinal fusion model
AU - Perdomo-Pantoja, Alexander
AU - Holmes, Christina
AU - Cottrill, Ethan
AU - Rindone, Alexandra N.
AU - Ishida, Wataru
AU - Taylor, Maritza
AU - Tomberlin, Colson
AU - Lo, Sheng Fu L.
AU - Grayson, Warren L.
AU - Witham, Timothy F.
N1 - Publisher Copyright:
© 2020 Wolters Kluwer Health, Inc. All rights reserved.
PY - 2021/5/15
Y1 - 2021/5/15
N2 - Study Design. Rat posterolateral lumbar fusion model. Objective. The aim of this study was to compare the efficacy of freshly isolated adipose tissue-derived stromal vascular fraction (A-SVF) and bone marrow cells (BMCs) cells in achieving spinal fusion in a rat model. Summary of Background Data. Adipose tissue-derived stromal cells (ASCs) offer advantages as a clinical cell source compared to bone marrow-derived stromal cells (BMSCs), including larger available tissue volumes and reduced donor site morbidity. While pre-clinical studies have shown that ex vivo expanded ASCs can be successfully used in spinal fusion, the use of A-SVF cells better allows for clinical translation. Methods. A-SVF cells were isolated from the inguinal fat pads, whereas BMCs were isolated from the long bones of syngeneic 6- to 8-week-old Lewis rats and combined with Vitoss (Stryker) bone graft substitute for subsequent transplantation. Posterolateral spinal fusion surgery at L4-L5 was performed on 36 female Lewis rats divided into three experimental groups: Vitoss bone graft substitute only (VO group); Vitoss þ 2.5 × 106 A-SVF cells/ side; and, Vitoss þ 2.5 × 106 BMCs/side. Fusion was assessed 8 weeks post-surgery via manual palpation, micro-computed tomography (mCT) imaging, and histology. Results. mCT imaging analyses revealed that fusion volumes and mCT fusion scores in the A-SVF group were significantly higher than in the VO group; however, they were not significantly different between the A-SVF group and the BMC group. The average manual palpation score was highest in the A-SVF group compared with the BMC and VO groups. Fusion masses arising from cell-seeded implants yielded better bone quality than nonseeded bone graft substitute. Conclusion. In a rat model, A-SVF cells yielded a comparable fusion mass volume and radiographic rate of fusion to BMCs when combined with a clinical-grade bone graft substitute. These results suggest the feasibility of using freshly isolated A-SVF cells in spinal fusion procedures.
AB - Study Design. Rat posterolateral lumbar fusion model. Objective. The aim of this study was to compare the efficacy of freshly isolated adipose tissue-derived stromal vascular fraction (A-SVF) and bone marrow cells (BMCs) cells in achieving spinal fusion in a rat model. Summary of Background Data. Adipose tissue-derived stromal cells (ASCs) offer advantages as a clinical cell source compared to bone marrow-derived stromal cells (BMSCs), including larger available tissue volumes and reduced donor site morbidity. While pre-clinical studies have shown that ex vivo expanded ASCs can be successfully used in spinal fusion, the use of A-SVF cells better allows for clinical translation. Methods. A-SVF cells were isolated from the inguinal fat pads, whereas BMCs were isolated from the long bones of syngeneic 6- to 8-week-old Lewis rats and combined with Vitoss (Stryker) bone graft substitute for subsequent transplantation. Posterolateral spinal fusion surgery at L4-L5 was performed on 36 female Lewis rats divided into three experimental groups: Vitoss bone graft substitute only (VO group); Vitoss þ 2.5 × 106 A-SVF cells/ side; and, Vitoss þ 2.5 × 106 BMCs/side. Fusion was assessed 8 weeks post-surgery via manual palpation, micro-computed tomography (mCT) imaging, and histology. Results. mCT imaging analyses revealed that fusion volumes and mCT fusion scores in the A-SVF group were significantly higher than in the VO group; however, they were not significantly different between the A-SVF group and the BMC group. The average manual palpation score was highest in the A-SVF group compared with the BMC and VO groups. Fusion masses arising from cell-seeded implants yielded better bone quality than nonseeded bone graft substitute. Conclusion. In a rat model, A-SVF cells yielded a comparable fusion mass volume and radiographic rate of fusion to BMCs when combined with a clinical-grade bone graft substitute. These results suggest the feasibility of using freshly isolated A-SVF cells in spinal fusion procedures.
KW - Adipose-derived stromal vascular fraction
KW - Bone marrow cells
KW - Mesenchymal stromal cell
KW - Rat posterolateral fusion model spinal fusion
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U2 - 10.1097/BRS.0000000000003709
DO - 10.1097/BRS.0000000000003709
M3 - Article
C2 - 32991510
AN - SCOPUS:85105697614
SN - 0362-2436
VL - 46
SP - 631
EP - 637
JO - Spine
JF - Spine
IS - 10
ER -