Comparison of four plating media for isolating Vibrio cholerae

G. K. Morris, M. H. Merson, I. Huq, A. K. Kibrya, Robert E Black

Research output: Contribution to journalArticle

Abstract

Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and TTG agars were the best selective media, whereas sucrose tellurite teepol agar was the poorest. Both TTG and sucrose tellurite teepol agars were good for use in follow-up serological tests, whereas only TTG agar could be used for follow-up oxidase tests. In our opinion, TTG agar has more advantages for cholera research laboratories routinely culturing large numbers of patients for cholera on a daily basis and where media needs can be accurately predicted. In contrast, in smaller clinical laboratories or in laboratories investigating epidemics, thiosulfate citrate bile salts sucrose agar is best because it is commercially available and easy to prepare and can be used to distinguish colonies of suspect V. cholerae from V. parahaemolyticus.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalJournal of Clinical Microbiology
Volume9
Issue number1
StatePublished - 1979
Externally publishedYes

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Vibrio cholerae
Agar
Taurocholic Acid
Sucrose
Gelatin
Thiosulfates
Bile Acids and Salts
Citric Acid
Vibrio parahaemolyticus
Cholera
tellurous acid
Serologic Tests
Oxidoreductases

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Morris, G. K., Merson, M. H., Huq, I., Kibrya, A. K., & Black, R. E. (1979). Comparison of four plating media for isolating Vibrio cholerae. Journal of Clinical Microbiology, 9(1), 79-83.

Comparison of four plating media for isolating Vibrio cholerae. / Morris, G. K.; Merson, M. H.; Huq, I.; Kibrya, A. K.; Black, Robert E.

In: Journal of Clinical Microbiology, Vol. 9, No. 1, 1979, p. 79-83.

Research output: Contribution to journalArticle

Morris, GK, Merson, MH, Huq, I, Kibrya, AK & Black, RE 1979, 'Comparison of four plating media for isolating Vibrio cholerae', Journal of Clinical Microbiology, vol. 9, no. 1, pp. 79-83.
Morris, G. K. ; Merson, M. H. ; Huq, I. ; Kibrya, A. K. ; Black, Robert E. / Comparison of four plating media for isolating Vibrio cholerae. In: Journal of Clinical Microbiology. 1979 ; Vol. 9, No. 1. pp. 79-83.
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