Comparison of extracted proteins of human stomach tumor and normal tissues with liquid chromatography-multistage mass spectrometry

Fuwen Luo, Dingyin Tao, Peng Zhao, Lingyi Zhang, Yujie Jia, Weibing Zhang

Research output: Contribution to journalArticle

Abstract

Screening of tumor markers by proteomic technology is the research focus and key of early diagnosis of stomach cancer study. Aiming at the complexity of the extracted proteins from biological tissue reversed-phase high performance liquid chromatography (RP-HPLC) was employed as one of the most efficient chromatographic methods. Based on the difference of hydrophobicity RP-HPLC separation was performed to reduce the complexity of stomach cancer tissue and normal tissue samples separately. By comparing the chromatograms different components were collected. The fractions with the retention times from 45 min to 47 min were digested and identified by liquid chromatography-multistage mass spectrometry (LC-MS/ MS). Nine common proteins were found in both tumor tissue and normal tissue. Six specific proteins were screened in normal tissue and seventeen specific proteins were found in tumor tissue under the same conditions. Two proteins with higher abundance in tumor tissue were selected for further investigation. These proteins provide more information for future drug target and drug pathway research by the analysis of biological information.

Original languageEnglish (US)
Pages (from-to)34-37
Number of pages4
JournalChinese Journal of Chromatography (Se Pu)
Volume28
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

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Liquid chromatography
Liquid Chromatography
Mass spectrometry
Tumors
Mass Spectrometry
Stomach
Tissue
Proteins
Neoplasms
High performance liquid chromatography
Reverse-Phase Chromatography
Stomach Neoplasms
High Pressure Liquid Chromatography
Hydrophobicity
Tumor Biomarkers
Hydrophobic and Hydrophilic Interactions
Early Detection of Cancer
Research
Pharmaceutical Preparations
Proteomics

Keywords

  • Cancer
  • Liquid chromatography-multistage mass spectrometry (LC-MS/MS)
  • Specific proteins
  • Stomach tissue

ASJC Scopus subject areas

  • Biochemistry
  • Chemical Engineering(all)
  • Analytical Chemistry
  • Electrochemistry
  • Organic Chemistry

Cite this

Comparison of extracted proteins of human stomach tumor and normal tissues with liquid chromatography-multistage mass spectrometry. / Luo, Fuwen; Tao, Dingyin; Zhao, Peng; Zhang, Lingyi; Jia, Yujie; Zhang, Weibing.

In: Chinese Journal of Chromatography (Se Pu), Vol. 28, No. 1, 01.2010, p. 34-37.

Research output: Contribution to journalArticle

Luo, Fuwen ; Tao, Dingyin ; Zhao, Peng ; Zhang, Lingyi ; Jia, Yujie ; Zhang, Weibing. / Comparison of extracted proteins of human stomach tumor and normal tissues with liquid chromatography-multistage mass spectrometry. In: Chinese Journal of Chromatography (Se Pu). 2010 ; Vol. 28, No. 1. pp. 34-37.
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