Comparison of endogenous murine leukemia virus proviral organization and RNA expression in 3-methylcholanthrene-induced and spontaneous thymis lymphomas in RF and AKR mice

Jeffrey M Chinsky, M. M. Goodenow, M. Jackson, F. Lilly, L. Leinwand, G. Childs

Research output: Contribution to journalArticle

Abstract

3-Methylcholanthrene-induced T-cell thymic lymphomas in RF mice were examined for involvement of murine leukemia virus (MuLV)-related sequences in leukemogenesis. Both the expression of MuLV-related RNA species and the organization of endogenous MuLV proviral DNA were analyzed. Of 27 primary tumors examined, only 5 exhibited elevated MuLV-related RNA species homologous to xenotropic specific env DNA. None of these RNA species hybridized with ecotropic p15E DNA sequences. Only two of these five tumors contained MuLV-like RNA species that hybridized with ecotropic MuLV long terminal repeat sequences, despite the probe's ability to detect both ecotropic MuLV and mink cell focus-inducing viral RNA. No muLV resembling mink cell focus-inducing virus whose expression could be correlated with lymphomagenesis was detected in either preleukemic thymocytes, primary 3-methylcholanthrene-induced thymic tumors, tumors passaged in vivo, or cell lines derived from tumors. Restriction endonuclease analysis of DNA from both primary tumors and cell lines failed to reveal either proviral DNA with recombinant env genes or rearrangement of endogenous MuLV proviruses. Therefore, chemically induced lymphomagenesis in RF mice appears different from the spontaneous lymphomagenic process in AKR mice with respect to the involvement of endogenous MuLV sequences.

Original languageEnglish (US)
Pages (from-to)94-99
Number of pages6
JournalJournal of Virology
Volume53
Issue number1
StatePublished - 1985
Externally publishedYes

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Murine leukemia virus
Inbred AKR Mouse
Murine Leukemia Viruses
Methylcholanthrene
lymphoma
Lymphoma
Gammaretrovirus
RNA
neoplasms
mice
terminal repeat sequences
mink
DNA
Terminal Repeat Sequences
cell lines
Tumor Cell Line
proviruses
restriction mapping
thymocytes
Mink Cell Focus-Inducing Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Comparison of endogenous murine leukemia virus proviral organization and RNA expression in 3-methylcholanthrene-induced and spontaneous thymis lymphomas in RF and AKR mice. / Chinsky, Jeffrey M; Goodenow, M. M.; Jackson, M.; Lilly, F.; Leinwand, L.; Childs, G.

In: Journal of Virology, Vol. 53, No. 1, 1985, p. 94-99.

Research output: Contribution to journalArticle

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AU - Childs, G.

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