Abstract
The detection and classification of human papillomavirus (HPV) by a consensus primer polymerase chain reaction (PCR) technique were compared with detection and classification by dot filter hybridization (DFH) and Southern transfer hybridization (STH). PCR detected HPV in 87% of specimens; the detection rates for DFH and STH were 51% and 49%, respectively. The specific HPV types detected by STH were also detected by PCR in 90% of specimens. However, 75% of the samples positive for unclassified HPV by STH were typed by PCR. PCR results were reproducible, as assessed by repeat analysis (96% agreement), by analysis of paired same-day specimens (89% agreement), and by interlaboratory analysis (88% agreement). PCR is a sensitive, specific, and reproducible test for HPV detection and classification in clinical and epidemiologic studies.
Original language | English (US) |
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Pages (from-to) | 1003-1006 |
Number of pages | 4 |
Journal | Journal of clinical microbiology |
Volume | 31 |
Issue number | 4 |
DOIs |
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State | Published - 1993 |
Externally published | Yes |
ASJC Scopus subject areas
- Microbiology (medical)