Comparison of dot filter hybridization, southern transfer hybridization, and polymerase chain reaction amplification for diagnosis of anal human papillomavirus infection

J. M. Kuypers, C. W. Critchlow, P. E. Gravitt, D. A. Vernon, J. B. Sayer, M. M. Manos, N. B. Kiviat

Research output: Contribution to journalComment/debatepeer-review

Abstract

The detection and classification of human papillomavirus (HPV) by a consensus primer polymerase chain reaction (PCR) technique were compared with detection and classification by dot filter hybridization (DFH) and Southern transfer hybridization (STH). PCR detected HPV in 87% of specimens; the detection rates for DFH and STH were 51% and 49%, respectively. The specific HPV types detected by STH were also detected by PCR in 90% of specimens. However, 75% of the samples positive for unclassified HPV by STH were typed by PCR. PCR results were reproducible, as assessed by repeat analysis (96% agreement), by analysis of paired same-day specimens (89% agreement), and by interlaboratory analysis (88% agreement). PCR is a sensitive, specific, and reproducible test for HPV detection and classification in clinical and epidemiologic studies.

Original languageEnglish (US)
Pages (from-to)1003-1006
Number of pages4
JournalJournal of clinical microbiology
Volume31
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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