Comparison of dendrimer-based macromolecular contrast agents for dynamic micro-magnetic resonance lymphangiography

Hisataka Kobayashi, Satomi Kawamoto, Peter L. Choyke, Noriko Sato, Michael V. Knopp, Robert A. Star, Thomas A. Waldmann, Yutaka Tagaya, Martin W. Brechbiel

Research output: Contribution to journalArticle

Abstract

Few methods are currently available to visualize the entire lymphatic system. A method known as micro-magnetic resonance lymphangiography (MRL), which employs a dendrimer-based MRI contrast agent (PAMAM-G8) and a clinical-grade 1.5T MRI instrument, was recently developed for use in mice. In the present study, three dendrimer-based MRI contrast agents (PAMAM-G8, DAB-G5, and PAMAM-G4) with different pharmacokinetic characteristics were compared to determine the best reagent to visualize the lymphatic system under physiological or pathological conditions. In addition, two established MRI contrast agents (Gadomer-17 and Gd-[DTPA]-dimeglumine (Magnevist)) were used as control agents. In experiments with mice, most of the deep lymphatic system was visualized by micro-MRL with all agents except Gd-[DTPA]-dimeglumine. PAMAM-G8 was best for visualizing lymphatic vessels, whereas DAB-G5 was better for visualizing lymph nodes. PAMAM-G4 was intermediate in character between PAMAM-G8 and DAB-G5, except in exhibiting a low background signal (especially in the liver). The lymphatic system was not clearly visualized with Gd-[DTPA]-dimeglumine; however, the lymph nodes were visualized with Gadomer-17, although not as well as with dendrimer-based agents. In conclusion, DAB-G5 and PAMAM-G4 can be used to identify lymph nodes and lymphatic vessels, respectively. Their rapid excretion makes these compounds potentially attractive for human use.

Original languageEnglish (US)
Pages (from-to)758-766
Number of pages9
JournalMagnetic resonance in medicine
Volume50
Issue number4
DOIs
StatePublished - Oct 1 2003

Keywords

  • Dendrimers
  • Lymphangiography
  • Lymphatic disorder
  • MRI contrast agents
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Kobayashi, H., Kawamoto, S., Choyke, P. L., Sato, N., Knopp, M. V., Star, R. A., Waldmann, T. A., Tagaya, Y., & Brechbiel, M. W. (2003). Comparison of dendrimer-based macromolecular contrast agents for dynamic micro-magnetic resonance lymphangiography. Magnetic resonance in medicine, 50(4), 758-766. https://doi.org/10.1002/mrm.10583