Akut orta kulak iltihabi örneklerinde streptococcus pneumoniae ve haemophilus influenzaef v\m saptanmasinda kültür ve gerçek zamanli pcr yöntemlerinin karşilaştirilmasi

Translated title of the contribution: Comparison of culture and real-tinne PCR methods in the detection of streptococcus pneumoniae and haemophilus influenzae in acute otitis media effusion specinnens

Özgen KöSeoĝlu Eser, Şehnaz Alp, Alper Ergin, Kaan Ipçi, Alpaslan Alp, Deniz Gür, Cülşen Hasçelik

Research output: Contribution to journalArticle

Abstract

Streptococcus pneumoniae and Haemophilus influenzae are the major etiologic agents of acute otitis media. This study was aimed to compare the detection rate of S.pneumoniae and H.influenzae by culture and real-time polymerase chain reaction (Rt-PCR) in the middie ear effusions of patients diagnosed as acute otitis media. A total of 60 middle ear effusion samples collected from children with acute otitis media were included In the study. The samples were inoculated and incubated in BACTEC Ped Plus blood culture bottles and BACTEC 9120 system (BD Diagnostic Systems, MD), respectively, and the isolates were identified by conventional methods. For the molecular diagnosis of H.influenzae and S.pneumoniae, ply pneumolysin gene and HIB capsule region, respectively were amplified by Rt-PCR (LightCycler, Roche Diagnostics, Germany). H.influenzae and S.pneumoniae were isolated from 5 (8.3%) and 3 (5%) of the patient samples with conventional culture methods, respectively. In addition in 11.6% of the samples other microorganisms (Staphylococcus epidermidis, Streptococcus intermedius, Streptococcus sanguinis, Moraxella catarrhalis, Pseudomonas aeruginosa, Candida albicans) were also isolated. On the other hand H.influenzae and S.pneumoniae vere detected in 38 (63.3%) and 24 (40%) of the samples with Rt-PCR, respectively. There was about eight fold increase in the detection frequency of H.influenzae and S.pneumoniae with Rt-PCR compared to culture methods. When culture was accepted as the gold standard method, the sensitivity, specificity and positive predictive value of Rt-PCR in the detection of H.influenzae and S.pneumoniae were estimated as 80%, 51% and 98.2%, respectively. As a result, Rt-PCR was shown to be a sensitive method and could be preferred for the rapid diagnosis of H.influenzae and S.pneumoniae in the etiological diagnosis of acute otitis media, especially in culture negative cases.

Original languageTurkish
Pages (from-to)676-681
Number of pages6
JournalMikrobiyoloji Bulteni
Volume46
Issue number4
StatePublished - Oct 1 2012

    Fingerprint

Keywords

  • Haemophilus influenzae
  • Middle ear effusion
  • Real-time PCR
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this