Comparison of competitive protein binding radioassay of cortisol to double isotope dilution and porter silber methods

Inese Z. Beitins, Maurice H. Shaw, Avinoam Kowarski, Clande J. Migeon

Research output: Contribution to journalArticlepeer-review

Abstract

The competitive protein binding radioassay of corticoids was performed on 4 pools of plasma, and the results compared to those obtained on the same pools by paper chromatography and protein binding, double isotope dilution, and colorimetric (Porter-Silber reaction) techniques. In the low and normal ranges, the colorimetric method was the only one significantly different, i.e., higher. In the high ranges the colorimetric method was significantly higher; while the paper chromatography and protein binding techniques were significantly lower than the protein binding radioassay for corticoids.

Original languageEnglish (US)
Pages (from-to)765-776
Number of pages12
JournalSteroids
Volume15
Issue number6
DOIs
StatePublished - Jun 1970

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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