Comparison of broth and filtration methods for culturing of intravenous fluids.

J. N. Longfield, P. Charache, E. L. Diamond, T. R. Townsend

Research output: Contribution to journalArticle

Abstract

Ninety-six specimens of intravenous fluid solutions (D5/025 NS) were inoculated with S. aureus, E. coli, P. aeruginosa, K. pneumoniae, E. agglomerans, or C. albicans in concentrations of .1, 1, 10, or 10(2) organisms/ml. They were cultured in tubes containing 5 ml of double enriched broth and after passage through a .45 mu pore membrane filter. After 24 hours of incubation, broth cultures were 68% as sensitive as the filter cultures (p less than .001). At the lowest concentration (.1 organism/ml) broth cultures wer only 45% as sensitive as the membrane filter technique after 24 hours of growth (p less than .001). Membrane filters provide a rapid method to accurately detect and quantitate the presence of microbial contamination even at very low levels of concentration. The simplicity and accuracy of the filtration method offers the clinician a valuable adjunct in managing suspected cases of intravenous fluid related sepsis.

Original languageEnglish (US)
Pages (from-to)397-400
Number of pages4
JournalInfection Control
Volume3
Issue number5
StatePublished - Sep 1982
Externally publishedYes

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Membranes
Sepsis
Pneumonia
Escherichia coli
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Longfield, J. N., Charache, P., Diamond, E. L., & Townsend, T. R. (1982). Comparison of broth and filtration methods for culturing of intravenous fluids. Infection Control, 3(5), 397-400.

Comparison of broth and filtration methods for culturing of intravenous fluids. / Longfield, J. N.; Charache, P.; Diamond, E. L.; Townsend, T. R.

In: Infection Control, Vol. 3, No. 5, 09.1982, p. 397-400.

Research output: Contribution to journalArticle

Longfield, JN, Charache, P, Diamond, EL & Townsend, TR 1982, 'Comparison of broth and filtration methods for culturing of intravenous fluids.', Infection Control, vol. 3, no. 5, pp. 397-400.
Longfield JN, Charache P, Diamond EL, Townsend TR. Comparison of broth and filtration methods for culturing of intravenous fluids. Infection Control. 1982 Sep;3(5):397-400.
Longfield, J. N. ; Charache, P. ; Diamond, E. L. ; Townsend, T. R. / Comparison of broth and filtration methods for culturing of intravenous fluids. In: Infection Control. 1982 ; Vol. 3, No. 5. pp. 397-400.
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