TY - JOUR
T1 - Comparison of broth and filtration methods for culturing of intravenous fluids.
AU - Longfield, J. N.
AU - Charache, P.
AU - Diamond, E. L.
AU - Townsend, T. R.
N1 - Copyright:
Copyright 2017 Medline is the source for the citation and abstract of this record.
PY - 1982
Y1 - 1982
N2 - Ninety-six specimens of intravenous fluid solutions (D5/025 NS) were inoculated with S. aureus, E. coli, P. aeruginosa, K. pneumoniae, E. agglomerans, or C. albicans in concentrations of .1, 1, 10, or 10(2) organisms/ml. They were cultured in tubes containing 5 ml of double enriched broth and after passage through a .45 mu pore membrane filter. After 24 hours of incubation, broth cultures were 68% as sensitive as the filter cultures (p less than .001). At the lowest concentration (.1 organism/ml) broth cultures wer only 45% as sensitive as the membrane filter technique after 24 hours of growth (p less than .001). Membrane filters provide a rapid method to accurately detect and quantitate the presence of microbial contamination even at very low levels of concentration. The simplicity and accuracy of the filtration method offers the clinician a valuable adjunct in managing suspected cases of intravenous fluid related sepsis.
AB - Ninety-six specimens of intravenous fluid solutions (D5/025 NS) were inoculated with S. aureus, E. coli, P. aeruginosa, K. pneumoniae, E. agglomerans, or C. albicans in concentrations of .1, 1, 10, or 10(2) organisms/ml. They were cultured in tubes containing 5 ml of double enriched broth and after passage through a .45 mu pore membrane filter. After 24 hours of incubation, broth cultures were 68% as sensitive as the filter cultures (p less than .001). At the lowest concentration (.1 organism/ml) broth cultures wer only 45% as sensitive as the membrane filter technique after 24 hours of growth (p less than .001). Membrane filters provide a rapid method to accurately detect and quantitate the presence of microbial contamination even at very low levels of concentration. The simplicity and accuracy of the filtration method offers the clinician a valuable adjunct in managing suspected cases of intravenous fluid related sepsis.
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U2 - 10.1017/S0195941700057362
DO - 10.1017/S0195941700057362
M3 - Article
C2 - 6813283
AN - SCOPUS:0020186453
SN - 0195-9417
VL - 3
SP - 397
EP - 400
JO - Infection control : IC
JF - Infection control : IC
IS - 5
ER -