Comparison of broth and filtration methods for culturing of intravenous fluids.

J. N. Longfield, P. Charache, E. L. Diamond, T. R. Townsend

Research output: Contribution to journalArticlepeer-review


Ninety-six specimens of intravenous fluid solutions (D5/025 NS) were inoculated with S. aureus, E. coli, P. aeruginosa, K. pneumoniae, E. agglomerans, or C. albicans in concentrations of .1, 1, 10, or 10(2) organisms/ml. They were cultured in tubes containing 5 ml of double enriched broth and after passage through a .45 mu pore membrane filter. After 24 hours of incubation, broth cultures were 68% as sensitive as the filter cultures (p less than .001). At the lowest concentration (.1 organism/ml) broth cultures wer only 45% as sensitive as the membrane filter technique after 24 hours of growth (p less than .001). Membrane filters provide a rapid method to accurately detect and quantitate the presence of microbial contamination even at very low levels of concentration. The simplicity and accuracy of the filtration method offers the clinician a valuable adjunct in managing suspected cases of intravenous fluid related sepsis.

Original languageEnglish (US)
Pages (from-to)397-400
Number of pages4
JournalInfection control : IC
Issue number5
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Medicine(all)

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