Kli̇ni̇k örneklerde mi̇kobakteri̇leri̇n saptanmasinda BBL Mi̇kobakteri̇ Üreme İndi̇katör Tüp (MGIT) yöntemi̇, BACTEC radyometri̇k si̇stemi̇ ve Lowenstein-Jensen besi̇yeri̇ni̇n karşilaştirilmasi

Translated title of the contribution: Comparison of BBL Mycobacteria Growth Indicator Tube (MGIT) method, BACTEC radiometric system and Lowenstein-Jensen media for the detection of mycobacteria in clinical specimens

Alpaslan Alp, Gülşen Hasçelik

Research output: Contribution to journalArticle

Abstract

The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processed clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.

Original languageTurkish
Pages (from-to)229-235
Number of pages7
JournalMikrobiyoloji Bulteni
Volume36
Issue number3-4
StatePublished - 2002
Externally publishedYes

Fingerprint

Mycobacterium
Growth
Bacteria
Incubators
Acids
Routine Diagnostic Tests
Culture Media
Microscopy
Tuberculosis

Keywords

  • BACTEC 460 TB system
  • Lowenstein-Jensen medium
  • Mycobacteria
  • Mycobacteria Growth Indicator Tube(MGIT)
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

@article{c9603102b40e4dce8ce13cd0a054601f,
title = "Kli̇ni̇k {\"o}rneklerde mi̇kobakteri̇leri̇n saptanmasinda BBL Mi̇kobakteri̇ {\"U}reme İndi̇kat{\"o}r T{\"u}p (MGIT) y{\"o}ntemi̇, BACTEC radyometri̇k si̇stemi̇ ve Lowenstein-Jensen besi̇yeri̇ni̇n karşilaştirilmasi",
abstract = "The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processed clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.",
keywords = "BACTEC 460 TB system, Lowenstein-Jensen medium, Mycobacteria, Mycobacteria Growth Indicator Tube(MGIT), Mycobacterium tuberculosis",
author = "Alpaslan Alp and G{\"u}lşen Has{\cc}elik",
year = "2002",
language = "Turkish",
volume = "36",
pages = "229--235",
journal = "Mikrobiyoloji Bulteni",
issn = "0374-9096",
publisher = "Ankara Microbiology Society",
number = "3-4",

}

TY - JOUR

T1 - Kli̇ni̇k örneklerde mi̇kobakteri̇leri̇n saptanmasinda BBL Mi̇kobakteri̇ Üreme İndi̇katör Tüp (MGIT) yöntemi̇, BACTEC radyometri̇k si̇stemi̇ ve Lowenstein-Jensen besi̇yeri̇ni̇n karşilaştirilmasi

AU - Alp, Alpaslan

AU - Hasçelik, Gülşen

PY - 2002

Y1 - 2002

N2 - The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processed clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.

AB - The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processed clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.

KW - BACTEC 460 TB system

KW - Lowenstein-Jensen medium

KW - Mycobacteria

KW - Mycobacteria Growth Indicator Tube(MGIT)

KW - Mycobacterium tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=0038509162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038509162&partnerID=8YFLogxK

M3 - Article

C2 - 12838655

AN - SCOPUS:0038509162

VL - 36

SP - 229

EP - 235

JO - Mikrobiyoloji Bulteni

JF - Mikrobiyoloji Bulteni

SN - 0374-9096

IS - 3-4

ER -