Comparison of antigen expression on normal urothelial cells in tissue section and tissue culture

M. Liebert, G. Wedemeyer, J. H.C. Chang, J. A. Stein, P. E. McKeever, T. E. Carey, A. Flint, Z. Steplewski, D. J. Buchsbaum, R. L. Wahl, H. B. Grossman

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Abstract

Antigenic characterization of urothelial cells cultured from normal adult ureter was performed. These cells were cultured using a simplified isolation and culture technique and a commercially available serum-free medium. The cells growing in these cultures had epithelioid morphology and normal quantities of DNA. The antigen expression on these cultured normal urothelial cells was evaluated using a panel of monoclonal antibodies: 5G6.4, AN43, URO-5, anti-keratin and anti-blood group antibodies, and 425 (anti-epidermal growth factor receptor). Lower levels of anti-A and AN43 binding on cultured cells were observed than are seen on urothelial cells in section of normal ureter, while the binding of anti-blood group H, 5G6.4, and URO-5 was unchanged. Binding of anti-epidermal growth factor receptor antibody 425 was improved if the cells were grown in medium lacking epidermal growth factor. These results confirm the urothelial origin of these cultured urothelial cells but indicate that some antigenic differences between cultured normal urothelial cells and urothelial cells in situ in the normal ureter exist.

Original languageEnglish (US)
Pages (from-to)1288-1292
Number of pages5
JournalJournal of Urology
Volume144
Issue number5
DOIs
StatePublished - Jan 1 1990

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ASJC Scopus subject areas

  • Urology

Cite this

Liebert, M., Wedemeyer, G., Chang, J. H. C., Stein, J. A., McKeever, P. E., Carey, T. E., Flint, A., Steplewski, Z., Buchsbaum, D. J., Wahl, R. L., & Grossman, H. B. (1990). Comparison of antigen expression on normal urothelial cells in tissue section and tissue culture. Journal of Urology, 144(5), 1288-1292. https://doi.org/10.1016/S0022-5347(17)39721-5