Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults: Effect of human immunodeficiency virus infection

Paul J. Converse, Stephen L. Jones, Jacquie Astemborski, David Vlahov, Neil M H Graham

Research output: Contribution to journalArticle

Abstract

A novel, whole blood interferon-γ, (IFN-γ) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-γ in plasma supernatants were determined by rapid ELISA. Participants were then administered the tuberculin skin test (TST) and tested for cutaneous anergy. The whole blood IFN-γ test agreed (89%-100%) with a positive TST in both HIV-seropositive and -seronegative subjects, but reactivity to PPD was more detectable by the whole blood assay among those with negative TSTs or anergy. TST induration diameter and IFN-γ responses were correlated (Spearman's ρ = .45, P = .0001), but both responses were blunted by HIV infection. In summary, tuberculin reactivity appears to be more detectable by the whole blood IFN-γ assay than by TST, and the assay requires no return visit for test reading.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalJournal of Infectious Diseases
Volume176
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Tuberculin Test
Tuberculin
Virus Diseases
Skin Tests
Interferons
HIV
Hematologic Tests
Drug Users
Mitogens
Mycobacterium tuberculosis
Reading
Enzyme-Linked Immunosorbent Assay
Skin
Proteins

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults : Effect of human immunodeficiency virus infection. / Converse, Paul J.; Jones, Stephen L.; Astemborski, Jacquie; Vlahov, David; Graham, Neil M H.

In: Journal of Infectious Diseases, Vol. 176, No. 1, 1997, p. 144-150.

Research output: Contribution to journalArticle

Converse, Paul J. ; Jones, Stephen L. ; Astemborski, Jacquie ; Vlahov, David ; Graham, Neil M H. / Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults : Effect of human immunodeficiency virus infection. In: Journal of Infectious Diseases. 1997 ; Vol. 176, No. 1. pp. 144-150.
@article{7a42b0a65e7044c985420943168c1755,
title = "Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults: Effect of human immunodeficiency virus infection",
abstract = "A novel, whole blood interferon-γ, (IFN-γ) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-γ in plasma supernatants were determined by rapid ELISA. Participants were then administered the tuberculin skin test (TST) and tested for cutaneous anergy. The whole blood IFN-γ test agreed (89{\%}-100{\%}) with a positive TST in both HIV-seropositive and -seronegative subjects, but reactivity to PPD was more detectable by the whole blood assay among those with negative TSTs or anergy. TST induration diameter and IFN-γ responses were correlated (Spearman's ρ = .45, P = .0001), but both responses were blunted by HIV infection. In summary, tuberculin reactivity appears to be more detectable by the whole blood IFN-γ assay than by TST, and the assay requires no return visit for test reading.",
author = "Converse, {Paul J.} and Jones, {Stephen L.} and Jacquie Astemborski and David Vlahov and Graham, {Neil M H}",
year = "1997",
language = "English (US)",
volume = "176",
pages = "144--150",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults

T2 - Effect of human immunodeficiency virus infection

AU - Converse, Paul J.

AU - Jones, Stephen L.

AU - Astemborski, Jacquie

AU - Vlahov, David

AU - Graham, Neil M H

PY - 1997

Y1 - 1997

N2 - A novel, whole blood interferon-γ, (IFN-γ) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-γ in plasma supernatants were determined by rapid ELISA. Participants were then administered the tuberculin skin test (TST) and tested for cutaneous anergy. The whole blood IFN-γ test agreed (89%-100%) with a positive TST in both HIV-seropositive and -seronegative subjects, but reactivity to PPD was more detectable by the whole blood assay among those with negative TSTs or anergy. TST induration diameter and IFN-γ responses were correlated (Spearman's ρ = .45, P = .0001), but both responses were blunted by HIV infection. In summary, tuberculin reactivity appears to be more detectable by the whole blood IFN-γ assay than by TST, and the assay requires no return visit for test reading.

AB - A novel, whole blood interferon-γ, (IFN-γ) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-γ in plasma supernatants were determined by rapid ELISA. Participants were then administered the tuberculin skin test (TST) and tested for cutaneous anergy. The whole blood IFN-γ test agreed (89%-100%) with a positive TST in both HIV-seropositive and -seronegative subjects, but reactivity to PPD was more detectable by the whole blood assay among those with negative TSTs or anergy. TST induration diameter and IFN-γ responses were correlated (Spearman's ρ = .45, P = .0001), but both responses were blunted by HIV infection. In summary, tuberculin reactivity appears to be more detectable by the whole blood IFN-γ assay than by TST, and the assay requires no return visit for test reading.

UR - http://www.scopus.com/inward/record.url?scp=0030948788&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030948788&partnerID=8YFLogxK

M3 - Article

C2 - 9207360

AN - SCOPUS:0030948788

VL - 176

SP - 144

EP - 150

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 1

ER -