TY - JOUR
T1 - Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults
T2 - Effect of human immunodeficiency virus infection
AU - Converse, Paul J.
AU - Jones, Stephen L.
AU - Astemborski, Jacquie
AU - Vlahov, David
AU - Graham, Neil M.H.
N1 - Funding Information:
Received 23 September 1996; revised 7 February 1997. Presented in part: ATS-TB Workshop on Rapid Diagnostic Tests for Tuberculosis, San Diego, 8 February 1996 (poster). Informed consent was obtained from patients, and human experimentation guidelines of Johns Hopkins University were followed in the conduct of this research. Financial support: NIH (DA-07061, DA-04334, and DA-08009); Centers for Disease Control Cooperative Agreement CCU300466-10. Reprints or correspondence (new affiliation): Dr. Neil M. H. Graham, Glaxo Wellcome, Inc., Five Moore Drive, Research Triangle Park, NC 27709.
PY - 1997
Y1 - 1997
N2 - A novel, whole blood interferon-γ, (IFN-γ) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-γ in plasma supernatants were determined by rapid ELISA. Participants were then administered the tuberculin skin test (TST) and tested for cutaneous anergy. The whole blood IFN-γ test agreed (89%-100%) with a positive TST in both HIV-seropositive and -seronegative subjects, but reactivity to PPD was more detectable by the whole blood assay among those with negative TSTs or anergy. TST induration diameter and IFN-γ responses were correlated (Spearman's ρ = .45, P = .0001), but both responses were blunted by HIV infection. In summary, tuberculin reactivity appears to be more detectable by the whole blood IFN-γ assay than by TST, and the assay requires no return visit for test reading.
AB - A novel, whole blood interferon-γ, (IFN-γ) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-γ in plasma supernatants were determined by rapid ELISA. Participants were then administered the tuberculin skin test (TST) and tested for cutaneous anergy. The whole blood IFN-γ test agreed (89%-100%) with a positive TST in both HIV-seropositive and -seronegative subjects, but reactivity to PPD was more detectable by the whole blood assay among those with negative TSTs or anergy. TST induration diameter and IFN-γ responses were correlated (Spearman's ρ = .45, P = .0001), but both responses were blunted by HIV infection. In summary, tuberculin reactivity appears to be more detectable by the whole blood IFN-γ assay than by TST, and the assay requires no return visit for test reading.
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U2 - 10.1086/514016
DO - 10.1086/514016
M3 - Article
C2 - 9207360
AN - SCOPUS:0030948788
SN - 0022-1899
VL - 176
SP - 144
EP - 150
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -