Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine

Ebba Nexø, Stanford I. Lamberg, Morley D. Hollenberg

Research output: Contribution to journalArticle

Abstract

A radioreceptor assay for human epidermal growth factor-urogastrone (EGF-URO) employing mouse EGF-URO as tracer and calibration standards and human placental receptor as binding protein is compared with a radioimmunoassay for human EGF-URO. Results employing the receptor assay indicated similar values for urinary concentrations (1 - 16nmol/l)for healthy control subjects and for patients with psoriasis. Lower values were obtained for bed-ridden patients with burns or patients recovering from orthopaedic surgery. The results obtained by radioimmunoassay were somewhat higher and correlated poorly (r = 0.5) with those obtained with the radioreceptor assay. The reason for this discrepancy is discussed.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalScandinavian Journal of Clinical and Laboratory Investigation
Volume41
Issue number6
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Epidermal Growth Factor
Radioimmunoassay
Assays
Radioligand Assay
Urine
Orthopedics
Burns
Psoriasis
Surgery
Calibration
Healthy Volunteers
Carrier Proteins

Keywords

  • Burns
  • Epidermal growth factor
  • Psoriasis
  • Radioimmunoassay
  • Radioreceptor assay
  • Urinary level of EGF-URO
  • Urogastrone

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

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