Comparison of a Commercial Reversed Passive Latex Agglutination Assay to an Enzyme Immunoassay for the Detection of Shiga Toxin-Producing Escherichia coli

K. C. Carroll, K. Adamson, K. Korgenski, A. Croft, R. Hankemeier, J. Daly, C. H. Park

Research output: Contribution to journalArticlepeer-review

Abstract

A multicenter study was performed to compare the performance of a prototypic reversed passive latex agglutination assay (VTEC Screen "Seiken"; Denka-Seiken, Japan) with the Premier EHEC Enzyme Immunoassay (Meridian Diagnostics, USA) for the detection of Shiga toxin in 554 diarrheal stool samples. Standard culture on sorbitol MacConkey agar and the use of latex agglutination reagents were included to identify the Escherichia coli O157, O26 and O111 serotypes. There was 99% agreement between the VTEC screen and enzyme immunoassay (kappa=0.823). Seventeen samples were positive for toxin by one or both assays. One toxin-positive sample using the enzyme immunoassay and four positive samples using the VTEC Screen could not be confirmed. Serotypes identified included: O157:H7 (n=8), O26 (n=2), O111 (n=1) and O45:H2 (n=1). The VTEC screen is easy to perform and comparable to the Meridian EHEC test for detection of Shiga toxin in clinical samples.

Original languageEnglish (US)
Pages (from-to)689-692
Number of pages4
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume22
Issue number11
DOIs
StatePublished - Nov 1 2003

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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