Comparison of 3 techniques of fat grafting and cell-supplemented lipotransfer in athymic rats: A pilot study

Alexandra Condé-Green, Iwen Wu, Ian Graham, Jemin J. Chae, Cinthia B. Drachenberg, Devinder P. Singh, Luther Holton, Sheri Slezak, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Background: Given the wide application of autologous fat grafting, a new emphasis on fat processing techniques has emerged in an effort to limit unpredictable degrees of resorption often seen with this procedure. With the growing interest in regenerative medicine, approaches to supplement fat grafts with adipose-derived stem cells are evolving in hopes of promoting vascularization and neoadipogenesis. Objective: The authors evaluated the outcomes of the most common processing techniques for fat grafting-decantation, washing, high-speed centrifugation-and stromal vascular cell-supplemented lipotransfer to determine which method yields a higher percentage of retention and better quality graft. Methods: A total of 32 subcutaneous injections of processed human lipoaspirate were carried out in 8 athymic rats. Each animal received all 4 processing conditions, with end points at 4, 8, and 12 weeks postinjection. Evaluation of graft survival included serial measurements of volume retention and histologic analysis. Results: At 12 weeks postinjection, cell-supplemented and centrifuged grafts showed the most consistent volume maintenance. Based on histologic analysis, cell-supplemented and washed grafts had higher scores of viability and vascularity, with the former presenting the least cystic necrosis and calcification as well as minimal inflammation. Conclusions: Cell-supplemented lipotransfer had optimal outcomes for graft retention, viability, and vascularity, while washing resulted in high viability with a less intensive process. High-speed centrifugation resulted in consistent volume retention but lower viability. Each of these approaches is ideal under different circumstances and contributes to the versatility and reliability of fat grafting.

Original languageEnglish (US)
Pages (from-to)713-721
Number of pages9
JournalAesthetic Surgery Journal
Volume33
Issue number5
DOIs
StatePublished - Jul 2013

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Nude Rats
Adipocytes
Fats
Transplants
Centrifugation
Regenerative Medicine
Graft Survival
Subcutaneous Injections
Stromal Cells
Blood Vessels
Necrosis
Stem Cells
Maintenance
Inflammation

Keywords

  • adipose tissue processing
  • autologous fat grafting
  • cell-supplemented lipotransfer
  • graft viability
  • stromal vascular fraction
  • volume retention

ASJC Scopus subject areas

  • Surgery

Cite this

Comparison of 3 techniques of fat grafting and cell-supplemented lipotransfer in athymic rats : A pilot study. / Condé-Green, Alexandra; Wu, Iwen; Graham, Ian; Chae, Jemin J.; Drachenberg, Cinthia B.; Singh, Devinder P.; Holton, Luther; Slezak, Sheri; Elisseeff, Jennifer Hartt.

In: Aesthetic Surgery Journal, Vol. 33, No. 5, 07.2013, p. 713-721.

Research output: Contribution to journalArticle

Condé-Green, Alexandra ; Wu, Iwen ; Graham, Ian ; Chae, Jemin J. ; Drachenberg, Cinthia B. ; Singh, Devinder P. ; Holton, Luther ; Slezak, Sheri ; Elisseeff, Jennifer Hartt. / Comparison of 3 techniques of fat grafting and cell-supplemented lipotransfer in athymic rats : A pilot study. In: Aesthetic Surgery Journal. 2013 ; Vol. 33, No. 5. pp. 713-721.
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AU - Drachenberg, Cinthia B.

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