Comparison of 13 acellular pertussis vaccines

Overview and serologic response

K. M. Edwards, B. D. Meade, M. D. Decker, G. F. Reed, M. B. Rennels, M. C. Steinhoff, E. L. Anderson, J. A. Englund, M. E. Pichichero, Maria Deloria Knoll, A. Deforest

Research output: Contribution to journalArticle

Abstract

Objective. To compare the immunogenicity of a licensed conventional whole- cell (WCL) and 13 diphtheria-tetanus-acellular pertussis (DTaP) vaccines that differed in source, method of manufacture, and included antigens; all vaccines included diphtheria and tetanus toxoids. Methods. Healthy infants were enrolled through six university-based vaccine and treatment evaluation units and were randomized to receive one of the study vaccines at 2, 4, and 6 months of age. Sera were obtained before the first immunization and 1 month after the third immunization and were analyzed for antibody to pertussis toxin (PT), filamentous hemagglutinin, fimbriae, pertactin, and diphtheria and tetanus toxins. Chinese hamster ovary cell toxin neutralization assays were performed, and levels of agglutinating antibodies were determined. Results. Of 2342 infants enrolled, 1942 contributed usable preimmunization and postimmunization serum specimens. Each vaccine produced significant increases in antibodies directed against the included antigens; postimmunization antibody titers differed significantly among the DTaP vaccines. For each evaluated antigen, the majority of DTaP vaccines produced antibody responses that equaled or exceeded those produced by WCL. For some antigens (eg, PT), mean antibody levels by vaccine correlated poorly with the quantity of antigen included in each vaccine; for others (eg, fimbriae), there was a close correlation. Conclusion. Although serologic correlates of pertussis immunity are not defined, it is clear that DTaP vaccines can stimulate immune responses that exceed those of licensed whole-cell vaccine with respect to the measured antibodies. Particularly for PT, immunogenicity seems to depend on factors in addition to antigen concentration, possibly including antigen derivation and formulation. No DTaP was most or least immunogenic with respect to all included antigens.

Original languageEnglish (US)
Pages (from-to)548-557
Number of pages10
JournalPediatrics
Volume96
Issue number3 II SUPPL.
StatePublished - 1995
Externally publishedYes

Fingerprint

Acellular Vaccines
Pertussis Vaccine
Diphtheria-Tetanus-acellular Pertussis Vaccines
Antigens
Vaccines
Antibodies
Pertussis Toxin
Whooping Cough
Immunization
Diphtheria Toxoid
Tetanus Toxin
Diphtheria Toxin
Tetanus Toxoid
Diphtheria
Tetanus
Hemagglutinins
Cricetulus
Serum
Antibody Formation
Ovary

Keywords

  • acellular
  • antibody
  • diphtheria-tetanus- pertussis
  • pertussis
  • vaccine
  • whole-cell
  • whooping cough

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Edwards, K. M., Meade, B. D., Decker, M. D., Reed, G. F., Rennels, M. B., Steinhoff, M. C., ... Deforest, A. (1995). Comparison of 13 acellular pertussis vaccines: Overview and serologic response. Pediatrics, 96(3 II SUPPL.), 548-557.

Comparison of 13 acellular pertussis vaccines : Overview and serologic response. / Edwards, K. M.; Meade, B. D.; Decker, M. D.; Reed, G. F.; Rennels, M. B.; Steinhoff, M. C.; Anderson, E. L.; Englund, J. A.; Pichichero, M. E.; Knoll, Maria Deloria; Deforest, A.

In: Pediatrics, Vol. 96, No. 3 II SUPPL., 1995, p. 548-557.

Research output: Contribution to journalArticle

Edwards, KM, Meade, BD, Decker, MD, Reed, GF, Rennels, MB, Steinhoff, MC, Anderson, EL, Englund, JA, Pichichero, ME, Knoll, MD & Deforest, A 1995, 'Comparison of 13 acellular pertussis vaccines: Overview and serologic response', Pediatrics, vol. 96, no. 3 II SUPPL., pp. 548-557.
Edwards KM, Meade BD, Decker MD, Reed GF, Rennels MB, Steinhoff MC et al. Comparison of 13 acellular pertussis vaccines: Overview and serologic response. Pediatrics. 1995;96(3 II SUPPL.):548-557.
Edwards, K. M. ; Meade, B. D. ; Decker, M. D. ; Reed, G. F. ; Rennels, M. B. ; Steinhoff, M. C. ; Anderson, E. L. ; Englund, J. A. ; Pichichero, M. E. ; Knoll, Maria Deloria ; Deforest, A. / Comparison of 13 acellular pertussis vaccines : Overview and serologic response. In: Pediatrics. 1995 ; Vol. 96, No. 3 II SUPPL. pp. 548-557.
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abstract = "Objective. To compare the immunogenicity of a licensed conventional whole- cell (WCL) and 13 diphtheria-tetanus-acellular pertussis (DTaP) vaccines that differed in source, method of manufacture, and included antigens; all vaccines included diphtheria and tetanus toxoids. Methods. Healthy infants were enrolled through six university-based vaccine and treatment evaluation units and were randomized to receive one of the study vaccines at 2, 4, and 6 months of age. Sera were obtained before the first immunization and 1 month after the third immunization and were analyzed for antibody to pertussis toxin (PT), filamentous hemagglutinin, fimbriae, pertactin, and diphtheria and tetanus toxins. Chinese hamster ovary cell toxin neutralization assays were performed, and levels of agglutinating antibodies were determined. Results. Of 2342 infants enrolled, 1942 contributed usable preimmunization and postimmunization serum specimens. Each vaccine produced significant increases in antibodies directed against the included antigens; postimmunization antibody titers differed significantly among the DTaP vaccines. For each evaluated antigen, the majority of DTaP vaccines produced antibody responses that equaled or exceeded those produced by WCL. For some antigens (eg, PT), mean antibody levels by vaccine correlated poorly with the quantity of antigen included in each vaccine; for others (eg, fimbriae), there was a close correlation. Conclusion. Although serologic correlates of pertussis immunity are not defined, it is clear that DTaP vaccines can stimulate immune responses that exceed those of licensed whole-cell vaccine with respect to the measured antibodies. Particularly for PT, immunogenicity seems to depend on factors in addition to antigen concentration, possibly including antigen derivation and formulation. No DTaP was most or least immunogenic with respect to all included antigens.",
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T1 - Comparison of 13 acellular pertussis vaccines

T2 - Overview and serologic response

AU - Edwards, K. M.

AU - Meade, B. D.

AU - Decker, M. D.

AU - Reed, G. F.

AU - Rennels, M. B.

AU - Steinhoff, M. C.

AU - Anderson, E. L.

AU - Englund, J. A.

AU - Pichichero, M. E.

AU - Knoll, Maria Deloria

AU - Deforest, A.

PY - 1995

Y1 - 1995

N2 - Objective. To compare the immunogenicity of a licensed conventional whole- cell (WCL) and 13 diphtheria-tetanus-acellular pertussis (DTaP) vaccines that differed in source, method of manufacture, and included antigens; all vaccines included diphtheria and tetanus toxoids. Methods. Healthy infants were enrolled through six university-based vaccine and treatment evaluation units and were randomized to receive one of the study vaccines at 2, 4, and 6 months of age. Sera were obtained before the first immunization and 1 month after the third immunization and were analyzed for antibody to pertussis toxin (PT), filamentous hemagglutinin, fimbriae, pertactin, and diphtheria and tetanus toxins. Chinese hamster ovary cell toxin neutralization assays were performed, and levels of agglutinating antibodies were determined. Results. Of 2342 infants enrolled, 1942 contributed usable preimmunization and postimmunization serum specimens. Each vaccine produced significant increases in antibodies directed against the included antigens; postimmunization antibody titers differed significantly among the DTaP vaccines. For each evaluated antigen, the majority of DTaP vaccines produced antibody responses that equaled or exceeded those produced by WCL. For some antigens (eg, PT), mean antibody levels by vaccine correlated poorly with the quantity of antigen included in each vaccine; for others (eg, fimbriae), there was a close correlation. Conclusion. Although serologic correlates of pertussis immunity are not defined, it is clear that DTaP vaccines can stimulate immune responses that exceed those of licensed whole-cell vaccine with respect to the measured antibodies. Particularly for PT, immunogenicity seems to depend on factors in addition to antigen concentration, possibly including antigen derivation and formulation. No DTaP was most or least immunogenic with respect to all included antigens.

AB - Objective. To compare the immunogenicity of a licensed conventional whole- cell (WCL) and 13 diphtheria-tetanus-acellular pertussis (DTaP) vaccines that differed in source, method of manufacture, and included antigens; all vaccines included diphtheria and tetanus toxoids. Methods. Healthy infants were enrolled through six university-based vaccine and treatment evaluation units and were randomized to receive one of the study vaccines at 2, 4, and 6 months of age. Sera were obtained before the first immunization and 1 month after the third immunization and were analyzed for antibody to pertussis toxin (PT), filamentous hemagglutinin, fimbriae, pertactin, and diphtheria and tetanus toxins. Chinese hamster ovary cell toxin neutralization assays were performed, and levels of agglutinating antibodies were determined. Results. Of 2342 infants enrolled, 1942 contributed usable preimmunization and postimmunization serum specimens. Each vaccine produced significant increases in antibodies directed against the included antigens; postimmunization antibody titers differed significantly among the DTaP vaccines. For each evaluated antigen, the majority of DTaP vaccines produced antibody responses that equaled or exceeded those produced by WCL. For some antigens (eg, PT), mean antibody levels by vaccine correlated poorly with the quantity of antigen included in each vaccine; for others (eg, fimbriae), there was a close correlation. Conclusion. Although serologic correlates of pertussis immunity are not defined, it is clear that DTaP vaccines can stimulate immune responses that exceed those of licensed whole-cell vaccine with respect to the measured antibodies. Particularly for PT, immunogenicity seems to depend on factors in addition to antigen concentration, possibly including antigen derivation and formulation. No DTaP was most or least immunogenic with respect to all included antigens.

KW - acellular

KW - antibody

KW - diphtheria-tetanus- pertussis

KW - pertussis

KW - vaccine

KW - whole-cell

KW - whooping cough

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