Comparison between the Gen-Probe transcription-mediated amplification Trichomonas vaginalis research assay and real-time PCR for Trichomonas vaginalis detection using a Roche LightCycler Instrument with female self-obtained vaginal swab samples and male urine samples

Andrew Hardick, Justin Hardick, Billie Jo Wood, Charlotte A Gaydos

Research output: Contribution to journalArticle

Abstract

This study compared two assays for Trichomonas vaginalis detection, Gen-Probe's transcription-mediated amplification (TMA) assay for Trichomonas vaginalis and BTUB FRET PCR, using self-obtained clinical samples from 611 patients. Infection status was defined as two positive results by two different tests. The initial TMA assay sensitivity was 96.7%; specificity was 97.5%. The TMA assay was comparable to BTUB FRET PCR.

Original languageEnglish (US)
Pages (from-to)4197-4199
Number of pages3
JournalJournal of Clinical Microbiology
Volume44
Issue number11
DOIs
StatePublished - Nov 2006

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Trichomonas vaginalis
Real-Time Polymerase Chain Reaction
Urine
Polymerase Chain Reaction
Research
Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

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abstract = "This study compared two assays for Trichomonas vaginalis detection, Gen-Probe's transcription-mediated amplification (TMA) assay for Trichomonas vaginalis and BTUB FRET PCR, using self-obtained clinical samples from 611 patients. Infection status was defined as two positive results by two different tests. The initial TMA assay sensitivity was 96.7{\%}; specificity was 97.5{\%}. The TMA assay was comparable to BTUB FRET PCR.",
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