TY - JOUR
T1 - Comparison between alpha-adrenergic- and K-opioidergic-mediated inositol(1,4,5)P3/inositol(1,3,4,5)P4 formation in adult cultured rat ventricular cardiomyocytes
AU - Ventura, Carlo
AU - Guarnieri, Carlo
AU - Stefanelli, Claudio
AU - Cirielli, Corrado
AU - Lakatta, Edward G.
AU - Capogrossi, Maurizio C.
PY - 1991/9/16
Y1 - 1991/9/16
N2 - In adult cultured rat ventricular cardiac myocytes, both the α-adrenergic agonist phenylephrine and the selective K opioid receptor ligand U-50,488H affected phosphoinositide turnover. Phenylephrine, over a time course of 10 min, caused a transient increase in Ins(1,4,5)P3 which peaked at 1 min and had returned to control at 2 min. In addition, phenylephrine produced a progressive and sustained increase in the formation of Ins(1,3,4, 5)P4 which achieved a plateau after 5 min of exposure to the agonist. U-50,488H induced an increase in Ins(1,4,5)P3 which peaked at 1 min at a level significantly higher than that due to phenylephrine and was still elevated after 10 min exposure to the K opioid receptor agonist. In addition, U-50,488H caused a sustained increase in Ins(1,3,4,5)P4 which was comparable to that due to phenylephrine. The stimulatory effects produced by phenylephrine and U-50,488H were receptor-mediated events, since they were fully antagonized by their respective antagonists, phentolamine or Mr-1452.
AB - In adult cultured rat ventricular cardiac myocytes, both the α-adrenergic agonist phenylephrine and the selective K opioid receptor ligand U-50,488H affected phosphoinositide turnover. Phenylephrine, over a time course of 10 min, caused a transient increase in Ins(1,4,5)P3 which peaked at 1 min and had returned to control at 2 min. In addition, phenylephrine produced a progressive and sustained increase in the formation of Ins(1,3,4, 5)P4 which achieved a plateau after 5 min of exposure to the agonist. U-50,488H induced an increase in Ins(1,4,5)P3 which peaked at 1 min at a level significantly higher than that due to phenylephrine and was still elevated after 10 min exposure to the K opioid receptor agonist. In addition, U-50,488H caused a sustained increase in Ins(1,3,4,5)P4 which was comparable to that due to phenylephrine. The stimulatory effects produced by phenylephrine and U-50,488H were receptor-mediated events, since they were fully antagonized by their respective antagonists, phentolamine or Mr-1452.
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U2 - 10.1016/0006-291X(91)91913-W
DO - 10.1016/0006-291X(91)91913-W
M3 - Article
C2 - 1898416
AN - SCOPUS:0025951104
VL - 179
SP - 972
EP - 978
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -