TY - JOUR
T1 - Comparison between a whole blood interferon-γ release assay and tuberculin skin testing for the detection of tuberculosis infection among patients at risk for tuberculosis exposure
AU - Kimura, Moriyo
AU - Converse, Paul J.
AU - Astemborski, Jacqueline
AU - Rothel, James S.
AU - Vlahov, David
AU - Comstock, George W.
AU - Graham, Neil M.H.
AU - Chaisson, Richard E.
AU - Bishai, William R.
N1 - Funding Information:
Received 26 June 1998; revised 4 December 1998. Presented in part: American Lung Association/American ThoracicSociety International Conference, Chicago, 24–29 April 1998 [poster]. Institutional review board approval was obtained prior to initiating this study, and informed consent was obtained from all patients participating. J.S.R. is an employee and stockholder of CSL, Ltd. Financial support: CDC (cooperative agreement U52-CCU300466-17); NIH (AI-36973, AI-37856, and HL-21670); CSL, Ltd. 1 Present addresses: Glaxo Wellcome Research and Development, Five Moore Dr., P.O. Box 13398, Research Triangle Park, NC, 27709 (N.M.H.G.); IGEN International, Inc., 16020 Industrial Dr., Gaithersburg, MD 20877 (P.J.C.). Reprints or correspondence: Dr. William Bishai, Johns Hopkins School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205 (wbishai@ jhsph.edu).
PY - 1999
Y1 - 1999
N2 - A new test that measures interferon-γ (IFN-γ) release in whole blood following stimulation with tuberculin has the potential to detect tuberculosis infection using a single blood draw. The IFN-γ release assay was compared with the standard tuberculin skin test (TST) among 467 intravenous drug users at risk for tuberculosis in urban Baltimore. Among 300 human immunodeficiency virus (HIV)-seronegative patients, the IFN-γ release assay was positive in 177 (59%), whereas the TST was positive in 71 (24%), for a percent agreement of 59% (κ = 26%). Among 167 HIV-seropositive subjects, the IFN-γ release assay identified 32 reactors (19%); the TST identified 16 reactors (9.6%), for a percent agreement of 82% (κ = 28%). The IFN-γ release assay detected more reactors than did the TST, but its agreement with TST was weak. As the TST is an imperfect standard, further evaluation of the IFN-γ release assay among uninfected persons and persons with culture-confirmed tuberculosis will be useful.
AB - A new test that measures interferon-γ (IFN-γ) release in whole blood following stimulation with tuberculin has the potential to detect tuberculosis infection using a single blood draw. The IFN-γ release assay was compared with the standard tuberculin skin test (TST) among 467 intravenous drug users at risk for tuberculosis in urban Baltimore. Among 300 human immunodeficiency virus (HIV)-seronegative patients, the IFN-γ release assay was positive in 177 (59%), whereas the TST was positive in 71 (24%), for a percent agreement of 59% (κ = 26%). Among 167 HIV-seropositive subjects, the IFN-γ release assay identified 32 reactors (19%); the TST identified 16 reactors (9.6%), for a percent agreement of 82% (κ = 28%). The IFN-γ release assay detected more reactors than did the TST, but its agreement with TST was weak. As the TST is an imperfect standard, further evaluation of the IFN-γ release assay among uninfected persons and persons with culture-confirmed tuberculosis will be useful.
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U2 - 10.1086/314707
DO - 10.1086/314707
M3 - Article
C2 - 10191241
AN - SCOPUS:0033008602
SN - 0022-1899
VL - 179
SP - 1297
EP - 1300
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -