Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah)

C. J. Morrow, B. A. Wolfe, T. L. Roth, D. E. Wildt, M. Bush, E. S. Blumer, M. W. Atkinson, S. L. Monfort

Research output: Contribution to journalArticle

Abstract

Ovarian response and pregnancy success in scimitar-horned oryx (n = 28) were compared, following treatment with two synchronization protocols and fixed-time artificial insemination (AI) with frozen-thawed semen. Each oryx received two injections of 500 μg of prostaglandin-F(2α) analogue (PGF(2α)-only) 11 days apart, and half received PGF(2α) in combination with an intravaginal progesterone-releasing device (CIDR11 + PGF(2α)). Semen was collected by electroejaculation from anaesthetised adult oryx and cryopreserved. Anaesthetised females were transcervically inseminated 56.0 ± 1.1 h (±S.E.M.) after PGF(2α) injection and/or device withdrawal using 28.0 ± 1.5 x 106 motile thawed sperm. Ovarian endocrine response was monitored in 20 females by analysing faecal oestrogen and progesterone metabolites. Periovulatory oestrogen peaks were detected in 19/20 (95%) females after synchronization. There were no between-treatment differences in oestrogen concentrations or peak characteristics (P > 0.05). Luteal development after synchronization was delayed in half the progesterone treated (CIDR11 + PGF(2α)) females, and faecal progestin excretion profiles indicated that the ovulatory follicle associated with synchronization either failed to ovulate or to fully lutenise. Pregnancy was diagnosed by ultrasonography and/or rectal palpation and was monitored by faecal progestin excretion. More (P = 0.013) pregnancies resulted from the PGF(2α)-only treatment (37.5%, 5/14) than from the CIDR11 + PGF(2α) treatment (0/14), and four healthy scimitar-horned oryx calves were born, three after gestation intervals of 247 days and one after 249 days. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)71-86
Number of pages16
JournalAnimal Reproduction Science
Volume59
Issue number1-2
DOIs
StatePublished - Apr 28 2000

Keywords

  • Artificial insemination
  • Faecal steroid
  • Ovulation induction
  • Scimitar-horned oryx
  • Ultrasound

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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    Morrow, C. J., Wolfe, B. A., Roth, T. L., Wildt, D. E., Bush, M., Blumer, E. S., Atkinson, M. W., & Monfort, S. L. (2000). Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah). Animal Reproduction Science, 59(1-2), 71-86. https://doi.org/10.1016/S0378-4320(00)00067-1