Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah)

C. J. Morrow; B. A. Wolfe; T. L. Roth; D. E. Wildt; M. Bush; E. S. Blumer; M. W. Atkinson; S. L. Monfort

doi:10.1016/S0378-4320(00)00067-1

Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah)

C. J. Morrow, B. A. Wolfe, T. L. Roth, D. E. Wildt, M. Bush, E. S. Blumer, M. W. Atkinson, S. L. Monfort

School of Medicine

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36 Scopus citations

Abstract

Ovarian response and pregnancy success in scimitar-horned oryx (n = 28) were compared, following treatment with two synchronization protocols and fixed-time artificial insemination (AI) with frozen-thawed semen. Each oryx received two injections of 500 μg of prostaglandin-F(2α) analogue (PGF(2α)-only) 11 days apart, and half received PGF(2α) in combination with an intravaginal progesterone-releasing device (CIDR11 + PGF(2α)). Semen was collected by electroejaculation from anaesthetised adult oryx and cryopreserved. Anaesthetised females were transcervically inseminated 56.0 ± 1.1 h (±S.E.M.) after PGF(2α) injection and/or device withdrawal using 28.0 ± 1.5 x 10⁶ motile thawed sperm. Ovarian endocrine response was monitored in 20 females by analysing faecal oestrogen and progesterone metabolites. Periovulatory oestrogen peaks were detected in 19/20 (95%) females after synchronization. There were no between-treatment differences in oestrogen concentrations or peak characteristics (P > 0.05). Luteal development after synchronization was delayed in half the progesterone treated (CIDR11 + PGF(2α)) females, and faecal progestin excretion profiles indicated that the ovulatory follicle associated with synchronization either failed to ovulate or to fully lutenise. Pregnancy was diagnosed by ultrasonography and/or rectal palpation and was monitored by faecal progestin excretion. More (P = 0.013) pregnancies resulted from the PGF(2α)-only treatment (37.5%, 5/14) than from the CIDR11 + PGF(2α) treatment (0/14), and four healthy scimitar-horned oryx calves were born, three after gestation intervals of 247 days and one after 249 days. (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	71-86
Number of pages	16
Journal	Animal Reproduction Science
Volume	59
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4320(00)00067-1
State	Published - Apr 28 2000

Keywords

Artificial insemination
Faecal steroid
Ovulation induction
Scimitar-horned oryx
Ultrasound

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
Endocrinology

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10.1016/S0378-4320(00)00067-1

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@article{1d90bdd568e24f249a97864ceff47068,

title = "Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah)",

abstract = "Ovarian response and pregnancy success in scimitar-horned oryx (n = 28) were compared, following treatment with two synchronization protocols and fixed-time artificial insemination (AI) with frozen-thawed semen. Each oryx received two injections of 500 μg of prostaglandin-F(2α) analogue (PGF(2α)-only) 11 days apart, and half received PGF(2α) in combination with an intravaginal progesterone-releasing device (CIDR11 + PGF(2α)). Semen was collected by electroejaculation from anaesthetised adult oryx and cryopreserved. Anaesthetised females were transcervically inseminated 56.0 ± 1.1 h (±S.E.M.) after PGF(2α) injection and/or device withdrawal using 28.0 ± 1.5 x 106 motile thawed sperm. Ovarian endocrine response was monitored in 20 females by analysing faecal oestrogen and progesterone metabolites. Periovulatory oestrogen peaks were detected in 19/20 (95%) females after synchronization. There were no between-treatment differences in oestrogen concentrations or peak characteristics (P > 0.05). Luteal development after synchronization was delayed in half the progesterone treated (CIDR11 + PGF(2α)) females, and faecal progestin excretion profiles indicated that the ovulatory follicle associated with synchronization either failed to ovulate or to fully lutenise. Pregnancy was diagnosed by ultrasonography and/or rectal palpation and was monitored by faecal progestin excretion. More (P = 0.013) pregnancies resulted from the PGF(2α)-only treatment (37.5%, 5/14) than from the CIDR11 + PGF(2α) treatment (0/14), and four healthy scimitar-horned oryx calves were born, three after gestation intervals of 247 days and one after 249 days. (C) 2000 Elsevier Science B.V.",

keywords = "Artificial insemination, Faecal steroid, Ovulation induction, Scimitar-horned oryx, Ultrasound",

author = "Morrow, {C. J.} and Wolfe, {B. A.} and Roth, {T. L.} and Wildt, {D. E.} and M. Bush and Blumer, {E. S.} and Atkinson, {M. W.} and Monfort, {S. L.}",

note = "Funding Information: We thank Dr. Christen Wemmer (Associate Director for Conservation, CRC) and Robert Reece, (Director of the Wilds) for supporting this collaborative research and providing the animals and facilities. We thank the staff of the Hoofstock, Veterinary, and Reproductive Programmes at CRC and the Wilds for animal husbandry, handling expertise, and technical assistance throughout this study. We also acknowledge the support of Dr. Geoff Birchard of George Mason University. Financial support of the Smithsonian Institution Scholarly Studies Program, Morris Animal Foundation, the Wilds, and George Mason University is gratefully acknowledged. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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doi = "10.1016/S0378-4320(00)00067-1",

language = "English (US)",

volume = "59",

pages = "71--86",

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T1 - Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah)

AU - Morrow, C. J.

AU - Wolfe, B. A.

AU - Roth, T. L.

AU - Wildt, D. E.

AU - Bush, M.

AU - Blumer, E. S.

AU - Atkinson, M. W.

AU - Monfort, S. L.

N1 - Funding Information: We thank Dr. Christen Wemmer (Associate Director for Conservation, CRC) and Robert Reece, (Director of the Wilds) for supporting this collaborative research and providing the animals and facilities. We thank the staff of the Hoofstock, Veterinary, and Reproductive Programmes at CRC and the Wilds for animal husbandry, handling expertise, and technical assistance throughout this study. We also acknowledge the support of Dr. Geoff Birchard of George Mason University. Financial support of the Smithsonian Institution Scholarly Studies Program, Morris Animal Foundation, the Wilds, and George Mason University is gratefully acknowledged. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2000/4/28

Y1 - 2000/4/28

N2 - Ovarian response and pregnancy success in scimitar-horned oryx (n = 28) were compared, following treatment with two synchronization protocols and fixed-time artificial insemination (AI) with frozen-thawed semen. Each oryx received two injections of 500 μg of prostaglandin-F(2α) analogue (PGF(2α)-only) 11 days apart, and half received PGF(2α) in combination with an intravaginal progesterone-releasing device (CIDR11 + PGF(2α)). Semen was collected by electroejaculation from anaesthetised adult oryx and cryopreserved. Anaesthetised females were transcervically inseminated 56.0 ± 1.1 h (±S.E.M.) after PGF(2α) injection and/or device withdrawal using 28.0 ± 1.5 x 106 motile thawed sperm. Ovarian endocrine response was monitored in 20 females by analysing faecal oestrogen and progesterone metabolites. Periovulatory oestrogen peaks were detected in 19/20 (95%) females after synchronization. There were no between-treatment differences in oestrogen concentrations or peak characteristics (P > 0.05). Luteal development after synchronization was delayed in half the progesterone treated (CIDR11 + PGF(2α)) females, and faecal progestin excretion profiles indicated that the ovulatory follicle associated with synchronization either failed to ovulate or to fully lutenise. Pregnancy was diagnosed by ultrasonography and/or rectal palpation and was monitored by faecal progestin excretion. More (P = 0.013) pregnancies resulted from the PGF(2α)-only treatment (37.5%, 5/14) than from the CIDR11 + PGF(2α) treatment (0/14), and four healthy scimitar-horned oryx calves were born, three after gestation intervals of 247 days and one after 249 days. (C) 2000 Elsevier Science B.V.

AB - Ovarian response and pregnancy success in scimitar-horned oryx (n = 28) were compared, following treatment with two synchronization protocols and fixed-time artificial insemination (AI) with frozen-thawed semen. Each oryx received two injections of 500 μg of prostaglandin-F(2α) analogue (PGF(2α)-only) 11 days apart, and half received PGF(2α) in combination with an intravaginal progesterone-releasing device (CIDR11 + PGF(2α)). Semen was collected by electroejaculation from anaesthetised adult oryx and cryopreserved. Anaesthetised females were transcervically inseminated 56.0 ± 1.1 h (±S.E.M.) after PGF(2α) injection and/or device withdrawal using 28.0 ± 1.5 x 106 motile thawed sperm. Ovarian endocrine response was monitored in 20 females by analysing faecal oestrogen and progesterone metabolites. Periovulatory oestrogen peaks were detected in 19/20 (95%) females after synchronization. There were no between-treatment differences in oestrogen concentrations or peak characteristics (P > 0.05). Luteal development after synchronization was delayed in half the progesterone treated (CIDR11 + PGF(2α)) females, and faecal progestin excretion profiles indicated that the ovulatory follicle associated with synchronization either failed to ovulate or to fully lutenise. Pregnancy was diagnosed by ultrasonography and/or rectal palpation and was monitored by faecal progestin excretion. More (P = 0.013) pregnancies resulted from the PGF(2α)-only treatment (37.5%, 5/14) than from the CIDR11 + PGF(2α) treatment (0/14), and four healthy scimitar-horned oryx calves were born, three after gestation intervals of 247 days and one after 249 days. (C) 2000 Elsevier Science B.V.

KW - Artificial insemination

KW - Faecal steroid

KW - Ovulation induction

KW - Scimitar-horned oryx

KW - Ultrasound

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Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah)

Abstract

Keywords

ASJC Scopus subject areas

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