Comparative ultrastructure of collagen fibrils in uterine leiomyomas and normal myometrium

Phyllis C. Leppert, Thomas Baginski, Cara Prupas, William H. Catherino, Susan Pletcher, James Segars

Research output: Contribution to journalArticle

Abstract

To examine the ultrastructural characteristics of extracellular matrix and mature collagen fibrils in uterine leiomyomas and compare them with those in adjacent normal myometrium. Analysis of paired leiomyoma-myometrium in surgical specimens. Research center and tertiary care center. Women undergoing medically indicated hysterectomy for symptomatic uterine leiomyomas. None. Appearance and spatial orientation of the collagen fibrils in leiomyomas compared with myometrium. Observation of specimens at x12,500 magnification indicated that collagen fibrils were more abundant, loosely packed, and arrayed in a nonparallel manner in leiomyomas compared with myometrium. Random areas were examined at x6,500 to x64,000 magnification and revealed collagen fibrils of equal diameter in both leiomyomas and myometrium. However, an ordered and regular barbed appearance was present in collagen fibrils from myometrium but was lacking in leiomyomas. Leiomyomas contain an abnormal collagen fibril structure and orientation, which suggests that the well-regulated fibril formation in myometrium is altered in leiomyomas. Alterations in collagen genes may play a role in the pathogenesis of leiomyomas.

Original languageEnglish (US)
Pages (from-to)1182-1187
Number of pages6
JournalFertility and Sterility
Volume82
Issue numberSUPPL. 3
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Myometrium
Leiomyoma
Collagen
Hysterectomy
Tertiary Care Centers
Extracellular Matrix
Observation

Keywords

  • collagen
  • collagen fibrillogenesis
  • electron microscopy
  • Leiomyomas

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Comparative ultrastructure of collagen fibrils in uterine leiomyomas and normal myometrium. / Leppert, Phyllis C.; Baginski, Thomas; Prupas, Cara; Catherino, William H.; Pletcher, Susan; Segars, James.

In: Fertility and Sterility, Vol. 82, No. SUPPL. 3, 10.2004, p. 1182-1187.

Research output: Contribution to journalArticle

Leppert, Phyllis C. ; Baginski, Thomas ; Prupas, Cara ; Catherino, William H. ; Pletcher, Susan ; Segars, James. / Comparative ultrastructure of collagen fibrils in uterine leiomyomas and normal myometrium. In: Fertility and Sterility. 2004 ; Vol. 82, No. SUPPL. 3. pp. 1182-1187.
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