Comparative ultrastructural morphology of human basophils stimulated to release histamine by anti-IgE, recombinant IgE-dependent histamine-releasing factor, or monocyte chemotactic protein-1

Ann M. Dvorak, John T. Schroeder, Donald W. MacGlashan, Karen P. Bryan, Ellen S. Morgan, Lawrence M. Lichtenstein, Susan M. MacDonald

Research output: Contribution to journalArticle

Abstract

An ultrastructural analysis of human basophils stimulated with anti- IgE, recombinant histamine-releasing factor (rHRF), or monocyte chemotactic protein-1 (MCP-1) (compared with unstimulated cells) was performed. Partially purified peripheral blood basophils were prepared for electron microscopy at time points known to precede histamine release and at half-maximum histamine times for each secretagogue. Activation morphologies associated with stimulation included granule-vesicle attachments, piecemeal degranulation, analytic degranulation, and uropod formation. These features were qualitatively similar in the stimulated samples. Quantitative differences were evident, however, when stimulated samples were compared with controls or at different time points after stimulation with a single agent or when individual secretagogues were compared. All stimulated samples differed quantitatively from the control samples. Rank orders for morphologic activation events revealed that the most trigger for anaphylactic degranulation was anti-IgE > MCP-1 > rHRF, whereas the most effective trigger for uropod formation was rHRF > anti-IgE > MCP-1. Rank orders for piecemeal degranulation and granule-vesicle attachments were the same; MCP-1 > anti- IgE > rHRF. Important relationships among these anatomic events reveal that the development of motile configurations is not associated with the development of secretion morphologies and that piecemeal degranulation precedes and is inversely related to anaphylactic degranulation in stimulated samples.

Original languageEnglish (US)
Pages (from-to)355-370
Number of pages16
JournalJournal of Allergy and Clinical Immunology
Volume98
Issue number2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Human basophil
  • anaphylactic degranulation
  • anti-IgE
  • electron microscopy
  • monocyte chemotactic protein-1
  • motile structures
  • piecemeal degranulation
  • recombinant IgE- dependent histamine-releasing factor
  • secretion
  • vesicle transport

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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