Comparative sialomics between hard and soft ticks: Implications for the evolution of blood-feeding behavior

Ben J. Mans, John F. Andersen, Ivo M.B. Francischetti, Jesus G. Valenzuela, Tom G. Schwan, Van M. Pham, Mark K. Garfield, Carl H. Hammer, José M.C. Ribeiro

Research output: Contribution to journalArticlepeer-review

Abstract

Ticks evolved various mechanisms to modulate their host's hemostatic and immune defenses. Differences in the anti-hemostatic repertoires suggest that hard and soft ticks evolved anti-hemostatic mechanisms independently, but raise questions on the conservation of salivary gland proteins in the ancestral tick lineage. To address this issue, the sialome (salivary gland secretory proteome) from the soft tick, Argas monolakensis, was determined by proteomic analysis and cDNA library construction of salivary glands from fed and unfed adult female ticks. The sialome is composed of ∼130 secretory proteins of which the most abundant protein folds are the lipocalin, BTSP, BPTI and metalloprotease families which also comprise the most abundant proteins found in the salivary glands. Comparative analysis indicates that the major protein families are conserved in hard and soft ticks. Phylogenetic analysis shows, however, that most gene duplications are lineage specific, indicating that the protein families analyzed possibly evolved most of their functions after divergence of the two major tick families. In conclusion, the ancestral tick may have possessed a simple (few members for each family), but diverse (many different protein families) salivary gland protein domain repertoire.

Original languageEnglish (US)
Pages (from-to)42-58
Number of pages17
JournalInsect Biochemistry and Molecular Biology
Volume38
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

Keywords

  • Argas
  • Blood-feeding
  • Evolution
  • Proteome
  • Sialome

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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