Abstract
Background: Primary biliary cirrhosis (PBC) is a liver specific chronic disease with unclear pathogenesis, especially for the early stage molecular events. The mitochondrion is a multi-functional organelle associated with various diseases including PBC. The purpose of this study was to discover the alterations in the mitochondria proteome using an early stage PBC mouse model for revealing the possible pathogenesis mechanisms in the early stages of PBC.Methods: Mouse model of early stage of PBC was constructed by consecutive administration of poly I:C. Mitochondria of mouse models and controls were purified and comparative proteomics was performed by iTRAQ technology. Then, differentially expressed proteins were validated by western blotting.Results: In total 354 proteins that satisfied the criteria for comparative proteomics study were identified. Of them, nine proteins were downregulated and 20 were up-regulated in liver mitochondria of PBC mouse model. Most differentially expressed proteins are associated with oxidation-reduction and lipid metabolism, and some are involved in the biosynthesis of steroid hormone and primary bile acid. Interestingly, four proteins (HCDH, CPT I, DECR, ECHDC2) involved in the fatty acid beta-oxidation were all upregulated.Conclusions: iTRAQ is a powerful tool for comparative proteomics study of PBC mouse model and differentially expressed proteins in mitochondria proteome of PBC mouse model provide insights for the pathogenesis mechanism at early stage of PBC.
| Original language | English (US) |
|---|---|
| Article number | 64 |
| Journal | BMC Gastroenterology |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 12 2013 |
| Externally published | Yes |
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Keywords
- iTRAQ
- Molecular pathogenesis
- Primary biliary cirrhosis
- Proteomics
ASJC Scopus subject areas
- Gastroenterology
Cite this
Comparative proteomics study on liver mitochondria of primary biliary cirrhosis mouse model. / Song, Guang; Hu, Chaojun; Zhu, Huishan; Li, Xi; Zhao, Liying; Zhou, Renfang; Zhang, Xuan; Zhang, Fengchun; Wu, Lin; Li, Yongzhe.
In: BMC Gastroenterology, Vol. 13, No. 1, 64, 12.04.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative proteomics study on liver mitochondria of primary biliary cirrhosis mouse model
AU - Song, Guang
AU - Hu, Chaojun
AU - Zhu, Huishan
AU - Li, Xi
AU - Zhao, Liying
AU - Zhou, Renfang
AU - Zhang, Xuan
AU - Zhang, Fengchun
AU - Wu, Lin
AU - Li, Yongzhe
PY - 2013/4/12
Y1 - 2013/4/12
N2 - Background: Primary biliary cirrhosis (PBC) is a liver specific chronic disease with unclear pathogenesis, especially for the early stage molecular events. The mitochondrion is a multi-functional organelle associated with various diseases including PBC. The purpose of this study was to discover the alterations in the mitochondria proteome using an early stage PBC mouse model for revealing the possible pathogenesis mechanisms in the early stages of PBC.Methods: Mouse model of early stage of PBC was constructed by consecutive administration of poly I:C. Mitochondria of mouse models and controls were purified and comparative proteomics was performed by iTRAQ technology. Then, differentially expressed proteins were validated by western blotting.Results: In total 354 proteins that satisfied the criteria for comparative proteomics study were identified. Of them, nine proteins were downregulated and 20 were up-regulated in liver mitochondria of PBC mouse model. Most differentially expressed proteins are associated with oxidation-reduction and lipid metabolism, and some are involved in the biosynthesis of steroid hormone and primary bile acid. Interestingly, four proteins (HCDH, CPT I, DECR, ECHDC2) involved in the fatty acid beta-oxidation were all upregulated.Conclusions: iTRAQ is a powerful tool for comparative proteomics study of PBC mouse model and differentially expressed proteins in mitochondria proteome of PBC mouse model provide insights for the pathogenesis mechanism at early stage of PBC.
AB - Background: Primary biliary cirrhosis (PBC) is a liver specific chronic disease with unclear pathogenesis, especially for the early stage molecular events. The mitochondrion is a multi-functional organelle associated with various diseases including PBC. The purpose of this study was to discover the alterations in the mitochondria proteome using an early stage PBC mouse model for revealing the possible pathogenesis mechanisms in the early stages of PBC.Methods: Mouse model of early stage of PBC was constructed by consecutive administration of poly I:C. Mitochondria of mouse models and controls were purified and comparative proteomics was performed by iTRAQ technology. Then, differentially expressed proteins were validated by western blotting.Results: In total 354 proteins that satisfied the criteria for comparative proteomics study were identified. Of them, nine proteins were downregulated and 20 were up-regulated in liver mitochondria of PBC mouse model. Most differentially expressed proteins are associated with oxidation-reduction and lipid metabolism, and some are involved in the biosynthesis of steroid hormone and primary bile acid. Interestingly, four proteins (HCDH, CPT I, DECR, ECHDC2) involved in the fatty acid beta-oxidation were all upregulated.Conclusions: iTRAQ is a powerful tool for comparative proteomics study of PBC mouse model and differentially expressed proteins in mitochondria proteome of PBC mouse model provide insights for the pathogenesis mechanism at early stage of PBC.
KW - iTRAQ
KW - Molecular pathogenesis
KW - Primary biliary cirrhosis
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=84876979613&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876979613&partnerID=8YFLogxK
U2 - 10.1186/1471-230X-13-64
DO - 10.1186/1471-230X-13-64
M3 - Article
C2 - 23586776
AN - SCOPUS:84876979613
VL - 13
JO - BMC Gastroenterology
JF - BMC Gastroenterology
SN - 1471-230X
IS - 1
M1 - 64
ER -