Comparative properties of five human ovarian adenocarcinoma cell lines

R. N. Buick, R. Pullano, J. M. Trent

Research output: Contribution to journalArticle

Abstract

We describe the derivation of three human ovarian carcinoma cell lines and the comparison of their properties with two previously described cell lines of like histology (SKOV-3 and CAOV-3). Two of the new lines (HOC-1 and HOC-7) were derived from separate ascites tumors (at 9-month intervals) of a patient with well-differentiated serous adenocarcinoma of the ovary. The third new line, HEY, was derived from a human ovarian cancer xenograft (HX-62) originally grown from a peritoneal deposit of a patient with moderately differentiated papillary cystadenocarcinoma of the ovary. The cell lines demonstrated differential ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice. Dose-response curves were generated for clonogenic cell survival of cells exposed to common chemotherapeutic agents; one of the lines (HEY) shows a degree of resistance to the alkylating agent cis-diamminedichloroplatinum(II) (cis-platinum). Common karyological features included structural abnormalities of chromosomes 3 and 11. Heterogeneity of expression of ovarian tumor-associated antigens was documented.

Original languageEnglish (US)
Pages (from-to)3668-3676
Number of pages9
JournalCancer Research
Volume45
Issue number8
StatePublished - 1985
Externally publishedYes

Fingerprint

Adenocarcinoma
Heterografts
Cell Line
Ovary
Papillary Cystadenocarcinoma
Inbred CBA Mouse
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 3
Alkylating Agents
Ascites
Ovarian Neoplasms
Cisplatin
Cell Survival
Histology
Carcinoma
Neoplasms
ovarian tumor associated antigen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Buick, R. N., Pullano, R., & Trent, J. M. (1985). Comparative properties of five human ovarian adenocarcinoma cell lines. Cancer Research, 45(8), 3668-3676.

Comparative properties of five human ovarian adenocarcinoma cell lines. / Buick, R. N.; Pullano, R.; Trent, J. M.

In: Cancer Research, Vol. 45, No. 8, 1985, p. 3668-3676.

Research output: Contribution to journalArticle

Buick, RN, Pullano, R & Trent, JM 1985, 'Comparative properties of five human ovarian adenocarcinoma cell lines', Cancer Research, vol. 45, no. 8, pp. 3668-3676.
Buick, R. N. ; Pullano, R. ; Trent, J. M. / Comparative properties of five human ovarian adenocarcinoma cell lines. In: Cancer Research. 1985 ; Vol. 45, No. 8. pp. 3668-3676.
@article{5388d9628e9a40e9a68513d681a36b9a,
title = "Comparative properties of five human ovarian adenocarcinoma cell lines",
abstract = "We describe the derivation of three human ovarian carcinoma cell lines and the comparison of their properties with two previously described cell lines of like histology (SKOV-3 and CAOV-3). Two of the new lines (HOC-1 and HOC-7) were derived from separate ascites tumors (at 9-month intervals) of a patient with well-differentiated serous adenocarcinoma of the ovary. The third new line, HEY, was derived from a human ovarian cancer xenograft (HX-62) originally grown from a peritoneal deposit of a patient with moderately differentiated papillary cystadenocarcinoma of the ovary. The cell lines demonstrated differential ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice. Dose-response curves were generated for clonogenic cell survival of cells exposed to common chemotherapeutic agents; one of the lines (HEY) shows a degree of resistance to the alkylating agent cis-diamminedichloroplatinum(II) (cis-platinum). Common karyological features included structural abnormalities of chromosomes 3 and 11. Heterogeneity of expression of ovarian tumor-associated antigens was documented.",
author = "Buick, {R. N.} and R. Pullano and Trent, {J. M.}",
year = "1985",
language = "English (US)",
volume = "45",
pages = "3668--3676",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "8",

}

TY - JOUR

T1 - Comparative properties of five human ovarian adenocarcinoma cell lines

AU - Buick, R. N.

AU - Pullano, R.

AU - Trent, J. M.

PY - 1985

Y1 - 1985

N2 - We describe the derivation of three human ovarian carcinoma cell lines and the comparison of their properties with two previously described cell lines of like histology (SKOV-3 and CAOV-3). Two of the new lines (HOC-1 and HOC-7) were derived from separate ascites tumors (at 9-month intervals) of a patient with well-differentiated serous adenocarcinoma of the ovary. The third new line, HEY, was derived from a human ovarian cancer xenograft (HX-62) originally grown from a peritoneal deposit of a patient with moderately differentiated papillary cystadenocarcinoma of the ovary. The cell lines demonstrated differential ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice. Dose-response curves were generated for clonogenic cell survival of cells exposed to common chemotherapeutic agents; one of the lines (HEY) shows a degree of resistance to the alkylating agent cis-diamminedichloroplatinum(II) (cis-platinum). Common karyological features included structural abnormalities of chromosomes 3 and 11. Heterogeneity of expression of ovarian tumor-associated antigens was documented.

AB - We describe the derivation of three human ovarian carcinoma cell lines and the comparison of their properties with two previously described cell lines of like histology (SKOV-3 and CAOV-3). Two of the new lines (HOC-1 and HOC-7) were derived from separate ascites tumors (at 9-month intervals) of a patient with well-differentiated serous adenocarcinoma of the ovary. The third new line, HEY, was derived from a human ovarian cancer xenograft (HX-62) originally grown from a peritoneal deposit of a patient with moderately differentiated papillary cystadenocarcinoma of the ovary. The cell lines demonstrated differential ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice. Dose-response curves were generated for clonogenic cell survival of cells exposed to common chemotherapeutic agents; one of the lines (HEY) shows a degree of resistance to the alkylating agent cis-diamminedichloroplatinum(II) (cis-platinum). Common karyological features included structural abnormalities of chromosomes 3 and 11. Heterogeneity of expression of ovarian tumor-associated antigens was documented.

UR - http://www.scopus.com/inward/record.url?scp=0021933379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021933379&partnerID=8YFLogxK

M3 - Article

C2 - 4016745

AN - SCOPUS:0021933379

VL - 45

SP - 3668

EP - 3676

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 8

ER -