TY - JOUR
T1 - Comparative molecular and immunoregulatory analysis of extracellular vesicles from Candida albicans and Candida auris.
AU - Zamith-Miranda, Daniel
AU - Heyman, Heino M.
AU - Couvillion, Sneha P.
AU - Cordero, Radames J.B.
AU - Rodrigues, Macio L.
AU - Nimrichter, Leonardo
AU - Casadevall, Arturo
AU - Amatuzzi, Rafaela F.
AU - Alves, Lysangela R.
AU - Nakayasu, Ernesto S.
AU - Nosanchuk, Joshua D.
N1 - Publisher Copyright:
The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/4
Y1 - 2020/11/4
N2 - Candida auris is a recently described multidrug-resistant pathogenic fungus that is increasingly responsible for healthcare associated outbreaks across the world. Bloodstream infections of this fungus cause death in up to 70% of the cases. Aggravating this scenario, C. auris’ disease-promoting mechanisms are poorly understood. Fungi release extracellular vesicles (EVs) carrying a broad range of molecules including proteins, lipids, carbohydrates, pigments, and RNA, many of which are virulence factors. Here, we carried out a comparative molecular characterization of C. auris and C. albicans EVs and evaluated their capacity to modulate effector mechanisms of host immune defense. Using proteomics, lipidomics, and transcriptomics, we found that C. auris released EVs with payloads that were strikingly different from EVs released by C. albicans. EVs released by C. auris potentiated the adhesion of this yeast to an epithelial cell monolayer. C. auris EVs also induced the expression of surface activation markers and cytokines by bone marrow-derived dendritic cells. Altogether, our findings show distinct profiles and properties of EVs released by C. auris and by C. albicans, and highlight the potential contribution of C. auris EVs to the pathogenesis of this emerging pathogen.
AB - Candida auris is a recently described multidrug-resistant pathogenic fungus that is increasingly responsible for healthcare associated outbreaks across the world. Bloodstream infections of this fungus cause death in up to 70% of the cases. Aggravating this scenario, C. auris’ disease-promoting mechanisms are poorly understood. Fungi release extracellular vesicles (EVs) carrying a broad range of molecules including proteins, lipids, carbohydrates, pigments, and RNA, many of which are virulence factors. Here, we carried out a comparative molecular characterization of C. auris and C. albicans EVs and evaluated their capacity to modulate effector mechanisms of host immune defense. Using proteomics, lipidomics, and transcriptomics, we found that C. auris released EVs with payloads that were strikingly different from EVs released by C. albicans. EVs released by C. auris potentiated the adhesion of this yeast to an epithelial cell monolayer. C. auris EVs also induced the expression of surface activation markers and cytokines by bone marrow-derived dendritic cells. Altogether, our findings show distinct profiles and properties of EVs released by C. auris and by C. albicans, and highlight the potential contribution of C. auris EVs to the pathogenesis of this emerging pathogen.
KW - Candida auris
KW - Candidiasis
KW - Extracellular vesicles
KW - Fungal pathogenesis
KW - Multi-omics
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U2 - 10.1101/2020.11.04.368472
DO - 10.1101/2020.11.04.368472
M3 - Article
AN - SCOPUS:85098779096
JO - Advances in Water Resources
JF - Advances in Water Resources
SN - 0309-1708
ER -