Comparative inter-strain sequence analysis of the putative regulatory region of murine psychostimulant-regulated gene GNB1 (G protein β1 subunit gene)

Nobue Kitanaka, Junichi Kitanaka, Donna Walther, Xiao Bing Wang, George R. Uhl

Research output: Contribution to journalArticle

Abstract

We isolated a cDNA clone from a murine genomic library of C57BL/6 strain, carrying 13.8 kb of nucleotides including exon 1 of heterotrimeric GTP-binding protein β1 subunit gene (genetic symbol, GNB1) and 10.6kb of the 5′ flanking region. Sequence comparison with GNB1 gene locus from 129Sv strain revealed a 0.2% divergence in a 13.2 kb common region between these two strains. The divergence consisted of eight single nucleotide polymorphisms, three insertions and one deletion, with 129Sv used as the reference. The exon 1 and the putative regulation elements, such as cyclic AMP response element, AP1, AP2, Sp1 and nuclear factor-κB recognition sites, were perfectly conserved. The expression of GNB1 mRNA was significantly increased in mouse striatum 2h after single methamphetamine administration with an approximately 150% expression level compared with the basal level. In contrast, no change in the expression level was observed in the cerebral cortex. After the chronic methamphetamine treatment regimen, the expression level of GNB1 mRNA did not change in any brain regions examined. These results suggest (1) that the 5′ flanking nucleotide sequence of GNB1 gene was strictly conserved for its possible contribution to the same change in the expression level between the mouse strains in response to psychostimulants and (2) that the initial process of development of behavioral sensitization appeared to occur parallel to the significant increase in the expression level of GNB1 gene in the mouse striatum.

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume14
Issue number4
DOIs
StatePublished - Aug 2003
Externally publishedYes

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Keywords

  • 5′ Flanking region
  • GNB1 gene
  • Inter-strain polymorphism
  • Methamphetamine
  • Promoter sequence
  • Psychostimulantregulated gene

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Endocrinology
  • Molecular Biology

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