Comparative integrated molecular analysis of intraocular medulloepitheliomas and central nervous system embryonal tumors with multilayered rosettes confirms that they are distinct nosologic entities

Andrey Korshunov, Frederick A. Jakobiec, Charles G Eberhart, Volker Hovestadt, David Capper, David T W Jones, Dominik Sturm, Anna M. Stagner, Deepak P. Edward, Ralph C. Eagle, Alan D. Proia, Arend Koch, Marina Ryzhova, Anastasia Ektova, Ulrich Schüller, Olga Zheludkova, Peter Lichter, Andreas von Deimling, Stefan M. Pfister, Marcel Kool

Research output: Contribution to journalArticle

Abstract

Intraocular medulloepithelioma (IO MEPL) is an uncommon embryonal neuroepithelial neoplasm of the eye. These ocular neoplasms have been compared with intracranial medulloepitheliomas or other histologic variants of CNS embryonal tumor with multilayered rosettes (CNS ETMR) due to their morphological mimicry. We performed comprehensive molecular analysis to explore the histogenetic and biologic relationships between 22 IO MEPL and 68 CNS ETMR. Routinely prepared paraffin-embedded samples were assessed for genome-wide methylation profiles using the Illumina Methylation 450k BeadChip array. We identified strong cytogenetic and epigenetic differences between ocular neoplasms and CNS ETMR. None of the IO MEPL cases displayed the ETMR-specific amplification of the C19MC locus. Instead, cytogenetic analysis of the IO MEPL showed numerous copy number aberrations which involved either whole chromosomes or chromosomal arms; recurrent aberrations in these tumors affected chromosomes 1p, 4, 8 and 16p. DNA methylation patterns were also strikingly different between these two tumor entities, suggesting that they do not share common origins and biological behaviors. Comparative cluster analysis of 198 pediatric CNS tumors and 22 IO MEPL revealed a clear demarcation of the CNS ETMR and IO MEPL profiles from other CNS entities. In conclusion, although IO MEPL shares some histopathological features with CNS ETMR, they manifest striking molecular diversity at the cytogenetic and epigenetic levels. Consequently they deserve a separate nosologic designation in future tumor classifications, where CNS MEPL could be designated as a histological variant of CNS ETMR.

Original languageEnglish (US)
Pages (from-to)538-544
Number of pages7
JournalNeuropathology
Volume35
Issue number6
DOIs
StatePublished - Dec 1 2015

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Primitive Neuroectodermal Tumors
Central Nervous System Neoplasms
Neoplasms
Eye Neoplasms
Epigenomics
Cytogenetics
Methylation
Neuroepithelial Neoplasms
Chromosomes, Human, Pair 4
Germ Cell and Embryonal Neoplasms
Cytogenetic Analysis
DNA Methylation
Paraffin
Cluster Analysis
Arm
Chromosomes
Genome
Pediatrics

Keywords

  • Brain
  • Chromosomal aberrations
  • Epigenetic
  • Eye
  • Medulloepithelioma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Comparative integrated molecular analysis of intraocular medulloepitheliomas and central nervous system embryonal tumors with multilayered rosettes confirms that they are distinct nosologic entities. / Korshunov, Andrey; Jakobiec, Frederick A.; Eberhart, Charles G; Hovestadt, Volker; Capper, David; Jones, David T W; Sturm, Dominik; Stagner, Anna M.; Edward, Deepak P.; Eagle, Ralph C.; Proia, Alan D.; Koch, Arend; Ryzhova, Marina; Ektova, Anastasia; Schüller, Ulrich; Zheludkova, Olga; Lichter, Peter; von Deimling, Andreas; Pfister, Stefan M.; Kool, Marcel.

In: Neuropathology, Vol. 35, No. 6, 01.12.2015, p. 538-544.

Research output: Contribution to journalArticle

Korshunov, A, Jakobiec, FA, Eberhart, CG, Hovestadt, V, Capper, D, Jones, DTW, Sturm, D, Stagner, AM, Edward, DP, Eagle, RC, Proia, AD, Koch, A, Ryzhova, M, Ektova, A, Schüller, U, Zheludkova, O, Lichter, P, von Deimling, A, Pfister, SM & Kool, M 2015, 'Comparative integrated molecular analysis of intraocular medulloepitheliomas and central nervous system embryonal tumors with multilayered rosettes confirms that they are distinct nosologic entities', Neuropathology, vol. 35, no. 6, pp. 538-544. https://doi.org/10.1111/neup.12227
Korshunov, Andrey ; Jakobiec, Frederick A. ; Eberhart, Charles G ; Hovestadt, Volker ; Capper, David ; Jones, David T W ; Sturm, Dominik ; Stagner, Anna M. ; Edward, Deepak P. ; Eagle, Ralph C. ; Proia, Alan D. ; Koch, Arend ; Ryzhova, Marina ; Ektova, Anastasia ; Schüller, Ulrich ; Zheludkova, Olga ; Lichter, Peter ; von Deimling, Andreas ; Pfister, Stefan M. ; Kool, Marcel. / Comparative integrated molecular analysis of intraocular medulloepitheliomas and central nervous system embryonal tumors with multilayered rosettes confirms that they are distinct nosologic entities. In: Neuropathology. 2015 ; Vol. 35, No. 6. pp. 538-544.
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AU - Jakobiec, Frederick A.

AU - Eberhart, Charles G

AU - Hovestadt, Volker

AU - Capper, David

AU - Jones, David T W

AU - Sturm, Dominik

AU - Stagner, Anna M.

AU - Edward, Deepak P.

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AU - Proia, Alan D.

AU - Koch, Arend

AU - Ryzhova, Marina

AU - Ektova, Anastasia

AU - Schüller, Ulrich

AU - Zheludkova, Olga

AU - Lichter, Peter

AU - von Deimling, Andreas

AU - Pfister, Stefan M.

AU - Kool, Marcel

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N2 - Intraocular medulloepithelioma (IO MEPL) is an uncommon embryonal neuroepithelial neoplasm of the eye. These ocular neoplasms have been compared with intracranial medulloepitheliomas or other histologic variants of CNS embryonal tumor with multilayered rosettes (CNS ETMR) due to their morphological mimicry. We performed comprehensive molecular analysis to explore the histogenetic and biologic relationships between 22 IO MEPL and 68 CNS ETMR. Routinely prepared paraffin-embedded samples were assessed for genome-wide methylation profiles using the Illumina Methylation 450k BeadChip array. We identified strong cytogenetic and epigenetic differences between ocular neoplasms and CNS ETMR. None of the IO MEPL cases displayed the ETMR-specific amplification of the C19MC locus. Instead, cytogenetic analysis of the IO MEPL showed numerous copy number aberrations which involved either whole chromosomes or chromosomal arms; recurrent aberrations in these tumors affected chromosomes 1p, 4, 8 and 16p. DNA methylation patterns were also strikingly different between these two tumor entities, suggesting that they do not share common origins and biological behaviors. Comparative cluster analysis of 198 pediatric CNS tumors and 22 IO MEPL revealed a clear demarcation of the CNS ETMR and IO MEPL profiles from other CNS entities. In conclusion, although IO MEPL shares some histopathological features with CNS ETMR, they manifest striking molecular diversity at the cytogenetic and epigenetic levels. Consequently they deserve a separate nosologic designation in future tumor classifications, where CNS MEPL could be designated as a histological variant of CNS ETMR.

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