Comparative genomic hybridization as part of a new diagnostic strategy in childhood hyperdiploid acute lymphoblastic leukemia

O. A. Haas, T. Henn, K. Romanakis, S. Du Manoir, C. Lengauer

Research output: Contribution to journalArticlepeer-review

Abstract

The detailed definition of karyotype changes associated with hyperdiploid acute lymphoblastic leukemia (ALL) is a precondition for their exploitation in minimal residual disease studies with fluorescence in situ hybridization analysis (FISH). In addition, certain karyotype patterns may have different prognostic implications. We have therefore used comparative genomic hybridization (CGH) to analyze the quantitative karyotype abnormalities in 14 cases of hyperdiploid ALL and correlated the results with those obtained by flow cytometry and conventional cytogenetic analyses. Despite an overall good agreement between the karyotypes obtained by classical banding techniques and CGH, we came across at least one karyotype discrepancy per case. Clarification of the discordant findings with fluorescence in situ hybridization (FISH) showed that all stem lines had been correctly defined by CGH. In eight cases, however, cytogenetic analyses revealed structural abnormalities that were undetectable by CGH. The other discrepancies were mainly due to a cytogenetic misinterpretation of similar sized and shaped chromosomes. Based on these findings we present a new diagnostic strategy for childhood ALL that includes flow cytometry and classical cytogenetics as well as CGH for the analysis of aneuploid cases and FISH to resolve the unavoidable discrepancies.

Original languageEnglish (US)
Pages (from-to)474-481
Number of pages8
JournalLeukemia
Volume12
Issue number4
DOIs
StatePublished - 1998

Keywords

  • Comparative genomic hybridization (CGH)
  • Cytogenetics
  • Flow cytometry
  • Fluorescence in situ hybridization (FISH)
  • Hyperdiploid ALL

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

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