Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster

Evgeny M. Zdobnov; Christian Von Mering; Ivica Letunic; David Torrents; Mikita Suyama; Richard R. Copley; George K. Christophides; Dana Thomasova; Robert A. Holt; G. Mani Subramanian; Hans Michael Mueller; George Dimopoulos; John H. Law; Michael A. Wells; Ewan Birney; Rosane Charlab; Aaron L. Halpern; Elena Kokoza; Cheryl L. Kraft; Zhongwu Lai; Suzanna Lewis; Christos Louis; Carolina Barillas-Mury; Deborah Nusskern; Gerald M. Rubin; Steven L. Salzberg; Granger G. Sutton; Pantelis Topalis; Ron Wides; Patrick Wincker; Mark Yandell; Frank H. Collins; Jose Ribeiro; William M. Gelbart; Fotis C. Kafatos; Peer Bork

doi:10.1126/science.1077061

Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster

Evgeny M. Zdobnov, Christian Von Mering, Ivica Letunic, David Torrents, Mikita Suyama, Richard R. Copley, George K. Christophides, Dana Thomasova, Robert A. Holt, G. Mani Subramanian, Hans Michael Mueller, George Dimopoulos, John H. Law, Michael A. Wells, Ewan Birney, Rosane Charlab, Aaron L. Halpern, Elena Kokoza, Cheryl L. Kraft, Zhongwu LaiSuzanna Lewis, Christos Louis, Carolina Barillas-Mury, Deborah Nusskern, Gerald M. Rubin, Steven L. Salzberg, Granger G. Sutton, Pantelis Topalis, Ron Wides, Patrick Wincker, Mark Yandell, Frank H. Collins, Jose Ribeiro, William M. Gelbart, Fotis C. Kafatos, Peer Bork

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Abstract

Comparison of the genomes and proteomes of the two diptera Anopheles gambiae and Drosophila melanogaster, which diverged about 250 million years ago, reveals considerable similarities. However, numerous differences are also observed; some of these must reflect the selection and subsequent adaptation associated with different ecologies and life strategies. Almost half of the genes in both genomes are interpreted as orthologs and show an average sequence identity of about 56%, which is slightly lower than that observed between the orthologs of the pufferfish and human (diverged about 450 million years ago). This indicates that these two insects diverged considerably faster than vertebrates. Aligned sequences reveal that orthologous genes have retained only half of their intron/exon structure, indicating that intron gains or losses have occurred at a rate of about one per gene per 125 million years. Chromosomal arms exhibit significant remnants of homology between the two species, although only 34% of the genes colocalize in small "microsyntenic" clusters, and major interarm transfers as well as intra-arm shuffling of gene order are detected.

Original language	English (US)
Pages (from-to)	149-159
Number of pages	11
Journal	Science
Volume	298
Issue number	5591
DOIs	https://doi.org/10.1126/science.1077061
State	Published - Oct 4 2002
Externally published	Yes

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Zdobnov, E. M., Von Mering, C., Letunic, I., Torrents, D., Suyama, M., Copley, R. R., Christophides, G. K., Thomasova, D., Holt, R. A., Subramanian, G. M., Mueller, H. M., Dimopoulos, G., Law, J. H., Wells, M. A., Birney, E., Charlab, R., Halpern, A. L., Kokoza, E., Kraft, C. L., ... Bork, P. (2002). Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster. Science, 298(5591), 149-159. https://doi.org/10.1126/science.1077061

Zdobnov, EM, Von Mering, C, Letunic, I, Torrents, D, Suyama, M, Copley, RR, Christophides, GK, Thomasova, D, Holt, RA, Subramanian, GM, Mueller, HM, Dimopoulos, G, Law, JH, Wells, MA, Birney, E, Charlab, R, Halpern, AL, Kokoza, E, Kraft, CL, Lai, Z, Lewis, S, Louis, C, Barillas-Mury, C, Nusskern, D, Rubin, GM, Salzberg, SL, Sutton, GG, Topalis, P, Wides, R, Wincker, P, Yandell, M, Collins, FH, Ribeiro, J, Gelbart, WM, Kafatos, FC & Bork, P 2002, 'Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster', Science, vol. 298, no. 5591, pp. 149-159. https://doi.org/10.1126/science.1077061

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title = "Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster",

abstract = "Comparison of the genomes and proteomes of the two diptera Anopheles gambiae and Drosophila melanogaster, which diverged about 250 million years ago, reveals considerable similarities. However, numerous differences are also observed; some of these must reflect the selection and subsequent adaptation associated with different ecologies and life strategies. Almost half of the genes in both genomes are interpreted as orthologs and show an average sequence identity of about 56%, which is slightly lower than that observed between the orthologs of the pufferfish and human (diverged about 450 million years ago). This indicates that these two insects diverged considerably faster than vertebrates. Aligned sequences reveal that orthologous genes have retained only half of their intron/exon structure, indicating that intron gains or losses have occurred at a rate of about one per gene per 125 million years. Chromosomal arms exhibit significant remnants of homology between the two species, although only 34% of the genes colocalize in small {"}microsyntenic{"} clusters, and major interarm transfers as well as intra-arm shuffling of gene order are detected.",

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AU - Zdobnov, Evgeny M.

AU - Von Mering, Christian

AU - Letunic, Ivica

AU - Torrents, David

AU - Suyama, Mikita

AU - Copley, Richard R.

AU - Christophides, George K.

AU - Thomasova, Dana

AU - Holt, Robert A.

AU - Subramanian, G. Mani

AU - Mueller, Hans Michael

AU - Dimopoulos, George

AU - Law, John H.

AU - Wells, Michael A.

AU - Birney, Ewan

AU - Charlab, Rosane

AU - Halpern, Aaron L.

AU - Kokoza, Elena

AU - Kraft, Cheryl L.

AU - Lai, Zhongwu

AU - Lewis, Suzanna

AU - Louis, Christos

AU - Barillas-Mury, Carolina

AU - Nusskern, Deborah

AU - Rubin, Gerald M.

AU - Salzberg, Steven L.

AU - Sutton, Granger G.

AU - Topalis, Pantelis

AU - Wides, Ron

AU - Wincker, Patrick

AU - Yandell, Mark

AU - Collins, Frank H.

AU - Ribeiro, Jose

AU - Gelbart, William M.

AU - Kafatos, Fotis C.

AU - Bork, Peer

PY - 2002/10/4

Y1 - 2002/10/4

N2 - Comparison of the genomes and proteomes of the two diptera Anopheles gambiae and Drosophila melanogaster, which diverged about 250 million years ago, reveals considerable similarities. However, numerous differences are also observed; some of these must reflect the selection and subsequent adaptation associated with different ecologies and life strategies. Almost half of the genes in both genomes are interpreted as orthologs and show an average sequence identity of about 56%, which is slightly lower than that observed between the orthologs of the pufferfish and human (diverged about 450 million years ago). This indicates that these two insects diverged considerably faster than vertebrates. Aligned sequences reveal that orthologous genes have retained only half of their intron/exon structure, indicating that intron gains or losses have occurred at a rate of about one per gene per 125 million years. Chromosomal arms exhibit significant remnants of homology between the two species, although only 34% of the genes colocalize in small "microsyntenic" clusters, and major interarm transfers as well as intra-arm shuffling of gene order are detected.

AB - Comparison of the genomes and proteomes of the two diptera Anopheles gambiae and Drosophila melanogaster, which diverged about 250 million years ago, reveals considerable similarities. However, numerous differences are also observed; some of these must reflect the selection and subsequent adaptation associated with different ecologies and life strategies. Almost half of the genes in both genomes are interpreted as orthologs and show an average sequence identity of about 56%, which is slightly lower than that observed between the orthologs of the pufferfish and human (diverged about 450 million years ago). This indicates that these two insects diverged considerably faster than vertebrates. Aligned sequences reveal that orthologous genes have retained only half of their intron/exon structure, indicating that intron gains or losses have occurred at a rate of about one per gene per 125 million years. Chromosomal arms exhibit significant remnants of homology between the two species, although only 34% of the genes colocalize in small "microsyntenic" clusters, and major interarm transfers as well as intra-arm shuffling of gene order are detected.

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ER -

Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster

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