Phenylalanine tRNA in Escherichia coli (15THU) is comprised of a known primary sequence of nucleotides. When cells are treated with chloramphenicol three forms of this tRNA appear, each separable by reversed phase chromatography (RPC-5). Using 32P labeling, these forms are compared by fingerprinting of their ribonuclease T1 digestion products with three different systems differing in the second dimension: high voltage electrophoresis, polyethylenimine-cellulose thin-layer chromatography, and homochromatography. Further compositional analysis shows that the three forms are identical, but that differences exist in the extent of modification of specific minor bases.
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