Comparative fingerprint and composition analysis of the three forms of32P-labeled phenylalanine tRNA from chloramphenicol-treated Escherichia coli

P C Huang, Michael B. Mann

Research output: Contribution to journalArticle

Abstract

Phenylalanine tRNA in Escherichia coli (15THU) is comprised of a known primary sequence of nucleotides. When cells are treated with chloramphenicol, three forms of this tRNA appear, each separable by reversed phase chromatography (RPC-5). Using 32P labeling, these forms are compared by fingerprinting of their ribonuclease T1 digestion products with three different systems differing in the second dimension: high voltage electrophoresis, polyethylenimine-cellulose thin-layer chromatography, and homochromatography. Further compositional analysis shows that the three forms are identical, but that differences exist in the extent of modification of specific minor bases.

Original languageEnglish (US)
Pages (from-to)4704-4710
Number of pages7
JournalBiochemistry®
Volume13
Issue number23
StatePublished - 1974

Fingerprint

Dermatoglyphics
Chloramphenicol
Transfer RNA
Phenylalanine
Escherichia coli
Ribonuclease T1
Polyethyleneimine
Thin layer chromatography
Reverse-Phase Chromatography
Thin Layer Chromatography
Chromatography
Electrophoresis
Chemical analysis
Cellulose
Labeling
Digestion
Nucleotides
Electric potential

ASJC Scopus subject areas

  • Biochemistry

Cite this

Comparative fingerprint and composition analysis of the three forms of32P-labeled phenylalanine tRNA from chloramphenicol-treated Escherichia coli. / Huang, P C; Mann, Michael B.

In: Biochemistry®, Vol. 13, No. 23, 1974, p. 4704-4710.

Research output: Contribution to journalArticle

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