TY - JOUR
T1 - Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation
AU - Du, Jian
AU - Tan, Elaine
AU - Kim, Hyo Jun
AU - Zhang, Allen
AU - Bhattacharya, Rahul
AU - Yarema, Kevin J.
N1 - Funding Information:
Funding for this study was provided by the National Institute for Biomedical Imaging and Bioengineering ( EB 005692 ).
PY - 2014
Y1 - 2014
N2 - Based on accumulating evidence that the 3D topography and the chemical features of a growth surface influence neuronal differentiation, we combined these two features by evaluating the cytotoxicity, proliferation, and differentiation of the rat PC12 line and human neural stem cells (hNSCs) on chitosan (CS), cellulose acetate (CA), and polyethersulfone (PES)-derived electrospun nanofibers that had similar diameters, centered in the 200-500 nm range. None of the nanofibrous materials were cytotoxic compared to 2D (e.g., flat surface) controls; however, proliferation generally was inhibited on the nanofibrous scaffolds although to a lesser extent on the polysaccharide-derived materials compared to PES. In an exception to the trend toward slower growth on the 3D substrates, hNSCs differentiated on the CS nanofibers proliferated faster than the 2D controls and both cell types showed enhanced indication of neuronal differentiation on the CS scaffolds. Together, these results demonstrate beneficial attributes of CS for neural tissue engineering when this polysaccharide is used in the context of the defined 3D topography found in electrospun nanofibers.
AB - Based on accumulating evidence that the 3D topography and the chemical features of a growth surface influence neuronal differentiation, we combined these two features by evaluating the cytotoxicity, proliferation, and differentiation of the rat PC12 line and human neural stem cells (hNSCs) on chitosan (CS), cellulose acetate (CA), and polyethersulfone (PES)-derived electrospun nanofibers that had similar diameters, centered in the 200-500 nm range. None of the nanofibrous materials were cytotoxic compared to 2D (e.g., flat surface) controls; however, proliferation generally was inhibited on the nanofibrous scaffolds although to a lesser extent on the polysaccharide-derived materials compared to PES. In an exception to the trend toward slower growth on the 3D substrates, hNSCs differentiated on the CS nanofibers proliferated faster than the 2D controls and both cell types showed enhanced indication of neuronal differentiation on the CS scaffolds. Together, these results demonstrate beneficial attributes of CS for neural tissue engineering when this polysaccharide is used in the context of the defined 3D topography found in electrospun nanofibers.
KW - Electrospun nanofibers
KW - Human neural stem cells
KW - Polysaccharides
KW - Rat PC12 cells
KW - Tissue engineering scaffolds
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U2 - 10.1016/j.carbpol.2013.08.050
DO - 10.1016/j.carbpol.2013.08.050
M3 - Article
C2 - 24274534
AN - SCOPUS:84884189177
SN - 0144-8617
VL - 99
SP - 483
EP - 490
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -