Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation

Jian Du, Elaine Tan, Hyo Jun Kim, Allen Zhang, Rahul Bhattacharya, Kevin J Yarema

Research output: Contribution to journalArticle

Abstract

Based on accumulating evidence that the 3D topography and the chemical features of a growth surface influence neuronal differentiation, we combined these two features by evaluating the cytotoxicity, proliferation, and differentiation of the rat PC12 line and human neural stem cells (hNSCs) on chitosan (CS), cellulose acetate (CA), and polyethersulfone (PES)-derived electrospun nanofibers that had similar diameters, centered in the 200-500 nm range. None of the nanofibrous materials were cytotoxic compared to 2D (e.g., flat surface) controls; however, proliferation generally was inhibited on the nanofibrous scaffolds although to a lesser extent on the polysaccharide-derived materials compared to PES. In an exception to the trend toward slower growth on the 3D substrates, hNSCs differentiated on the CS nanofibers proliferated faster than the 2D controls and both cell types showed enhanced indication of neuronal differentiation on the CS scaffolds. Together, these results demonstrate beneficial attributes of CS for neural tissue engineering when this polysaccharide is used in the context of the defined 3D topography found in electrospun nanofibers.

Original languageEnglish (US)
Pages (from-to)483-490
Number of pages8
JournalCarbohydrate Polymers
Volume99
DOIs
StatePublished - 2014

Fingerprint

Chitosan
Nanofibers
Scaffolds
Cellulose
Polysaccharides
Stem cells
Topography
Control surfaces
Cytotoxicity
Tissue engineering
Rats
polyether sulfone
acetylcellulose
Substrates

Keywords

  • Electrospun nanofibers
  • Human neural stem cells
  • Polysaccharides
  • Rat PC12 cells
  • Tissue engineering scaffolds

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation. / Du, Jian; Tan, Elaine; Kim, Hyo Jun; Zhang, Allen; Bhattacharya, Rahul; Yarema, Kevin J.

In: Carbohydrate Polymers, Vol. 99, 2014, p. 483-490.

Research output: Contribution to journalArticle

Du, Jian ; Tan, Elaine ; Kim, Hyo Jun ; Zhang, Allen ; Bhattacharya, Rahul ; Yarema, Kevin J. / Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation. In: Carbohydrate Polymers. 2014 ; Vol. 99. pp. 483-490.
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