Comparative characterisation of Russell's viper (Daboia/Vipera russelli) venoms from different regions of the Indian peninsula

Nijaguna B. Prasad, B. Uma, Subramanya K.G. Bhatt, Veerabasappa T. Gowda

Research output: Contribution to journalArticlepeer-review

Abstract

Russell's viper (Daboia/Vipera russelli) venom from different regions of India was subjected to chromatographic, electrophoretic, biochemical and immunological analysis. The elution profiles from ion-exchange chromatography and protein banding pattern from SDS-PAGE showed a significant variation in the constituents of venoms. The acidic proteins are found to be predominant in the venoms of eastern and western regions while basic proteins are the major contributors of the northern and southern regional venoms. The major variation of phospholipases A2 in the venom samples of India may be described as: southern regional venom is rich in basic, toxic PLA2 while this activity showed a dramatic decrease as one moves towards west, north and eastern regions of India. In addition, the caseinolytic, TAME-hydrolytic, anticoagulant, oedema-inducing and haemorrhagic activities of the venoms have also varied from one region to another. The muscle specimens of mice injected with venoms of different regions showed variable change in the muscle fibre damage and cell morphology. The eastern regional venom is most lethal among all the venoms. The lethal potencies for four regional venoms vary as: eastern>western>southern>northern. The polyclonal antibodies prepared against the venom of southern region showed cross-reaction with the venoms of other regions, but the extent of cross-reaction and diffusion patterns are different. However, the polyclonal antibodies prepared against southern regional venom showed no protection against lethal toxicity of other regional venoms. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)121-136
Number of pages16
JournalBiochimica et Biophysica Acta - General Subjects
Volume1428
Issue number2-3
DOIs
StatePublished - Aug 5 1999

Keywords

  • (Daboia/Vipera russelli)
  • Phospholipase A
  • Polyclonal antiserum
  • Venom variation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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