Comparative antioxidant potential of anaesthetics and perioperative drugs in vitro

David Mantle, Fadel Eddeb, Kiri Areni, Christopher Snowden, A. David Mendelow

Research output: Contribution to journalArticlepeer-review

Abstract

We have investigated the comparative antioxidant capacity of a range of anaesthetics (inhaled and intravenous) and perioperative neurosurgical drugs (at clinically relevant concentrations) using different radical species and assay methods in vitro. The highest levels of antioxidant activity against the ABTS(·+) radical were obtained with propofol (100 mmol/LTE) and dopamine (1080 mmol/LTE), respectively. However, only dopamine (12 mmol/l) showed antioxidant activity in protecting proteins in normal brain tissue from oxidative damage (assessed via SDS-PAGE analysis) induced by OH· or O2/(- ·) generated radiolytically in vitro. Neither dopamine nor propofol showed antioxidant activity against O2/(-·) generated chemically via reaction between xanthine and xanthine oxidase in vitro. From these data, together with data on the relative antioxidant properties of anaesthetics/drugs obtained by other research groups which we have reviewed, we conclude that the apparent antioxidant activity of a given compound may depend entirely on the free radical species and/or the method of generation or assay employed. Finally, we suggest that on the basis of data obtained showing protection of brain proteins from oxidative damage induced by OH·, or O2/(-·) in vitro, further investigation into the in vivo antioxidant therapeutic potential of dopamine (or its analogues) on neurosurgical patients may be warranted. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)41-53
Number of pages13
JournalClinica Chimica Acta
Volume301
Issue number1-2
DOIs
StatePublished - Nov 2000

Keywords

  • Anaesthetics
  • Antioxidants
  • Free radicals
  • Perioperative drugs

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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