Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi

A. Espinel-Ingroff, K. Dawson, M. Pfaller, E. Anaissie, B. Breslin, D. Dixon, A. Fothergill, V. Paetznick, J. Peter, M. Rinaldi, T. Walsh

Research output: Contribution to journalArticle

Abstract

The purpose of the study was to evaluate the interlaboratory agreement of broth dilution susceptibility methods for five species of conidium-forming (size range, 2 to 7 μm) filamentous fungi. The methods used included both macro- and microdilution methods that were adaptations of the proposed reference method of the National Committee for Clinical Laboratory Standards for yeasts (m27-P). The MICs of amphotericin B, fluconazole, itraconazole, miconazole, and ketoconazole were determined in six centers by both macro- and microdilution tests for 25 isolates of Aspergillus flavus, Aspergillus fumigatus, Pseudallescheria boydii, Rhizopus arrhizus, and Sporothrix schenckii. All isolates produced clearly detectable growth within 1 to 4 days at 35Γ in the RPMI 1640 medium. Colony counts of 0.4 x 106 to 3.3 x 106 CFU/ml (mean, 1.4 x 106 CFU/ml) were demonstrated in 90% of the 148 inoculum preparations. Overall, good intralaboratory agreement was demonstrated with amphotericin B, fluconazole, and ketoconazole MICs (90 to 97%). The agreement was lower with itraconazole MICs (59 to 79% median). Interlaboratory reproducibility demonstrated similar results: 90 to 100% agreement with amphotericin B, fluconazole, miconazole, and ketoconazole MICs and 59 to 91% with itraconazole MICs. Among the species tested, the MICs for S. schenckii showed the highest variability. The results of the study imply that it may be possible to develop a reference method for antifungal susceptibility testing of filamentous fungi.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume39
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Fungi
Ketoconazole
Itraconazole
Fluconazole
Amphotericin B
Miconazole
Pseudallescheria
Sporothrix
Rhizopus
Aspergillus flavus
Aspergillus fumigatus
Fungal Spores
Yeasts
Growth

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Espinel-Ingroff, A., Dawson, K., Pfaller, M., Anaissie, E., Breslin, B., Dixon, D., ... Walsh, T. (1995). Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. Antimicrobial Agents and Chemotherapy, 39(2), 314-319.

Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. / Espinel-Ingroff, A.; Dawson, K.; Pfaller, M.; Anaissie, E.; Breslin, B.; Dixon, D.; Fothergill, A.; Paetznick, V.; Peter, J.; Rinaldi, M.; Walsh, T.

In: Antimicrobial Agents and Chemotherapy, Vol. 39, No. 2, 1995, p. 314-319.

Research output: Contribution to journalArticle

Espinel-Ingroff, A, Dawson, K, Pfaller, M, Anaissie, E, Breslin, B, Dixon, D, Fothergill, A, Paetznick, V, Peter, J, Rinaldi, M & Walsh, T 1995, 'Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi', Antimicrobial Agents and Chemotherapy, vol. 39, no. 2, pp. 314-319.
Espinel-Ingroff, A. ; Dawson, K. ; Pfaller, M. ; Anaissie, E. ; Breslin, B. ; Dixon, D. ; Fothergill, A. ; Paetznick, V. ; Peter, J. ; Rinaldi, M. ; Walsh, T. / Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. In: Antimicrobial Agents and Chemotherapy. 1995 ; Vol. 39, No. 2. pp. 314-319.
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AU - Dawson, K.

AU - Pfaller, M.

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AU - Breslin, B.

AU - Dixon, D.

AU - Fothergill, A.

AU - Paetznick, V.

AU - Peter, J.

AU - Rinaldi, M.

AU - Walsh, T.

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