TY - JOUR
T1 - Comparative anatomy of the mammalian corneal subbasal nerve plexus
AU - Marfurt, Carl
AU - Anokwute, Miracle C.
AU - Fetcko, Kaleigh
AU - Mahony-Perez, Erin
AU - Farooq, Hassan
AU - Ross, Emily
AU - Baumanis, Maraya M.
AU - Weinberg, Rachel L.
AU - McCarron, Megan E.
AU - Mankowski, Joseph L.
N1 - Funding Information:
Supported by Summer Research Internship funding from Indiana University School of Medicine-Northwest-Gary (C.M.), National Institutes of Health Grants R01NS097221 (J.L.M) and U42OD013117 to support the macaque breeding colony (J.L.M)
Funding Information:
Supported by Summer Research Internship funding from Indiana University School of Medicine-Northwest-Gary (C.M.), National Institutes of Health Grants R01NS097221 (J.L.M) and U42OD013117 to support the macaque breeding colony (J.L.M) Disclosure: C. Marfurt, None; M.C. Anokwute, None; K. Fetcko, None; E. Mahony-Perez, None; H. Farooq, None; E. Ross,
Publisher Copyright:
© 2019 The Authors iovs.arvojournals.org j ISSN: 1552-5783
PY - 2019/12/1
Y1 - 2019/12/1
N2 - PURPOSE: The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe in detail the SNP architecture in eight different mammals, including several popular animal models used in cornea research. METHODS: Corneal nerves in mouse, rat, guinea pig, rabbit, dog, macaque, domestic pig, and cow eyes were stained immunohistochemically with antiserum directed against neurotubulin. SNP architecture was documented by digital photomicrography and large-scale reconstructions, that is, corneal nerve maps, using a drawing tube attached to a light microscope. RESULTS: Subbasal nerve fibers (SNFs) in mice, rats, guinea pigs, dogs, and macaques radiated centrally from the corneoscleral limbus toward the corneal apex in a whorl-like or spiraling pattern. SNFs in rabbit and bovine corneas swept horizontally across the ocular surface in a temporal-to-nasal direction and converged on the inferonasal limbus without forming a spiral. SNFs in the pig cornea radiated centrifugally in all directions, like a starburst, from a focal point located equidistant between the corneal apex and the superior pole. CONCLUSIONS: The results of the present study have demonstrated for the first time substantial interspecies differences in the architectural organization of the mammalian SNP. The physiological significance of these different patterns and the mechanisms that regulate SNP pattern formation in the mammalian cornea remain incompletely understood and warrant additional investigation.
AB - PURPOSE: The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe in detail the SNP architecture in eight different mammals, including several popular animal models used in cornea research. METHODS: Corneal nerves in mouse, rat, guinea pig, rabbit, dog, macaque, domestic pig, and cow eyes were stained immunohistochemically with antiserum directed against neurotubulin. SNP architecture was documented by digital photomicrography and large-scale reconstructions, that is, corneal nerve maps, using a drawing tube attached to a light microscope. RESULTS: Subbasal nerve fibers (SNFs) in mice, rats, guinea pigs, dogs, and macaques radiated centrally from the corneoscleral limbus toward the corneal apex in a whorl-like or spiraling pattern. SNFs in rabbit and bovine corneas swept horizontally across the ocular surface in a temporal-to-nasal direction and converged on the inferonasal limbus without forming a spiral. SNFs in the pig cornea radiated centrifugally in all directions, like a starburst, from a focal point located equidistant between the corneal apex and the superior pole. CONCLUSIONS: The results of the present study have demonstrated for the first time substantial interspecies differences in the architectural organization of the mammalian SNP. The physiological significance of these different patterns and the mechanisms that regulate SNP pattern formation in the mammalian cornea remain incompletely understood and warrant additional investigation.
KW - Corneal innervation
KW - Corneal nerves
KW - Ocular nerves
KW - Subbasal nerves
KW - Trigeminal nerve
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U2 - 10.1167/iovs.19-28519
DO - 10.1167/iovs.19-28519
M3 - Article
C2 - 31790560
AN - SCOPUS:85075916919
SN - 0146-0404
VL - 60
SP - 4972
EP - 4984
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 15
ER -