Comparative Analysis of Hemagglutination Inhibition Titers Generated Using Temporally Matched Serum and Plasma Samples

Gabriel N. Defang, Nicholas J. Martin, Timothy H. Burgess, Eugene V. Millar, LeNae A. Pecenka, Janine R. Danko, John C. Arnold, Tadeusz J. Kochel, Thomas C. Luke

Research output: Contribution to journalArticle

Abstract

Influenza-specific hemaggluitination inhibition (HAI) antibody titer, an indicator of immunity to influenza, is often used to measure exposure to influenza in surveillance and immunogenicity studies. Traditionally, serum has been the specimen of choice for HAI assays, but a desire to reduce the amount of blood collected during studies and the availability of plasma in archived sample collections warrant the evaluation of plasma for HAI titer. Therefore, the relationship between serum and plasma HAI titer values is of great interest. Here, we compare HAI titers determined on temporally matched serum and plasma (citrated and heparinized) using influenza A and B viruses. Bland-Altman plots, McNemar's test, and geometric coefficient of variation were used respectively for evaluating agreement, correlation and variability in the serum-plasma titer results. We observed a high degree of agreement (80.5%-98.8%) and correlation (r = 0.796-0.964) in the serum and matched plasma titer values although plasma titers were generally lower than corresponding serum titers. Calculated seropositive (HAI ≥40) rates were higher using serum titers than with plasma titers, but seroconversion rates were unaffected by sample type. Stronger agreement and decreased variability in titers were seen between serum and citrated plasma than between serum and heparinized plasma. Overall, these data suggest that serum or plasma can be used in serodiagnostic HAI assays, but seropositive rates may be underestimated using plasma HAI titers. The type of anticoagulant present in plasma may affect HAI titer values and warrants further investigation.

Original languageEnglish (US)
Article numbere48229
JournalPLoS One
Volume7
Issue number12
DOIs
StatePublished - Dec 20 2012
Externally publishedYes

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Hemagglutination
hemagglutination
blood serum
Plasmas
Serum
sampling
influenza
Human Influenza
Influenza B virus
Assays
anticoagulants
seroconversion
assays
Influenza A virus
immunity
Viruses
immune response
Anticoagulants
Immunity
Blood

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Defang, G. N., Martin, N. J., Burgess, T. H., Millar, E. V., Pecenka, L. A., Danko, J. R., ... Luke, T. C. (2012). Comparative Analysis of Hemagglutination Inhibition Titers Generated Using Temporally Matched Serum and Plasma Samples. PLoS One, 7(12), [e48229]. https://doi.org/10.1371/journal.pone.0048229

Comparative Analysis of Hemagglutination Inhibition Titers Generated Using Temporally Matched Serum and Plasma Samples. / Defang, Gabriel N.; Martin, Nicholas J.; Burgess, Timothy H.; Millar, Eugene V.; Pecenka, LeNae A.; Danko, Janine R.; Arnold, John C.; Kochel, Tadeusz J.; Luke, Thomas C.

In: PLoS One, Vol. 7, No. 12, e48229, 20.12.2012.

Research output: Contribution to journalArticle

Defang, GN, Martin, NJ, Burgess, TH, Millar, EV, Pecenka, LA, Danko, JR, Arnold, JC, Kochel, TJ & Luke, TC 2012, 'Comparative Analysis of Hemagglutination Inhibition Titers Generated Using Temporally Matched Serum and Plasma Samples', PLoS One, vol. 7, no. 12, e48229. https://doi.org/10.1371/journal.pone.0048229
Defang, Gabriel N. ; Martin, Nicholas J. ; Burgess, Timothy H. ; Millar, Eugene V. ; Pecenka, LeNae A. ; Danko, Janine R. ; Arnold, John C. ; Kochel, Tadeusz J. ; Luke, Thomas C. / Comparative Analysis of Hemagglutination Inhibition Titers Generated Using Temporally Matched Serum and Plasma Samples. In: PLoS One. 2012 ; Vol. 7, No. 12.
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