Comparative analysis of DNA content in polyomavirus-infected urothelial cells, urothelial dysplasia and high grade transitional cell carcinoma

Eva M. Wojcik, M. Craig Miller, Bradley C. Wright, Robert W. Veltri, Gerard J. O'Dowd

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To characterize the DNA content pattern in cytologically confirmed polyomavirus (PV)-infected urothelial cells and to compare it with DNA ploidy changes in cytologically diagnosed urothelial dysplasia and high grade transitional cell carcinoma. STUDY DESIGN: We selected 200 bladder cytology specimens consisting of four groups with 50 patients each in the following cytologic categories: (1) no evidence of malignancy or dysplasia (controls), (2) PV, (3) urothelial dysplasia (UD), and (4) high grade transitional cell carcinoma (TCC-HG). For each case, two slides with 25-ram filter imprints were stained, one using the Papanicolaou method and the other using the Feulgen staining method. The DNA index (DI), proliferative activity (S+G2M) and degree of hyperploidy (> 5C) were evaluated using an image analysis system. RESULTS: Using the Wilcoxon rank-sum test, statistically significant differences in the DI were found between the PV and UD groups (P = .008) and between the PV and TCC-HG groups (P <.0001). There was no significant difference in the DI between the PV and control groups. The S+G2M fraction for the PV group significantly differed from the control, UD and TCC-HG groups (all P<.0001). Between all four groups, the degrees of hyperploidy were significantly different as well (all P <.0001). CONCLUSION: Cytologically confirmed PV-infected urothelial cells demonstrated a unique DNA content pat- tern with mildly elevated proliferative activity and a significantly dispersed hyperploid DNA content pattern. DNA analysis can help to differentiate PV infection from dysplasia and high grade carcinoma.

Original languageEnglish (US)
Pages (from-to)430-436
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Volume19
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Polyomavirus
Transitional Cell Carcinoma
DNA
Nonparametric Statistics
Polyomavirus Infections
Charadriiformes
Ploidies
Cell Biology
Urinary Bladder
Staining and Labeling
Carcinoma
Control Groups

Keywords

  • Bladder neoplasm
  • Carcinoma
  • DNA
  • Ploidies
  • Polyomavirus
  • Transitional cell
  • Urothelium

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Cell Biology

Cite this

Comparative analysis of DNA content in polyomavirus-infected urothelial cells, urothelial dysplasia and high grade transitional cell carcinoma. / Wojcik, Eva M.; Miller, M. Craig; Wright, Bradley C.; Veltri, Robert W.; O'Dowd, Gerard J.

In: Analytical and Quantitative Cytology and Histology, Vol. 19, No. 5, 1997, p. 430-436.

Research output: Contribution to journalArticle

Wojcik, Eva M. ; Miller, M. Craig ; Wright, Bradley C. ; Veltri, Robert W. ; O'Dowd, Gerard J. / Comparative analysis of DNA content in polyomavirus-infected urothelial cells, urothelial dysplasia and high grade transitional cell carcinoma. In: Analytical and Quantitative Cytology and Histology. 1997 ; Vol. 19, No. 5. pp. 430-436.
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abstract = "OBJECTIVE: To characterize the DNA content pattern in cytologically confirmed polyomavirus (PV)-infected urothelial cells and to compare it with DNA ploidy changes in cytologically diagnosed urothelial dysplasia and high grade transitional cell carcinoma. STUDY DESIGN: We selected 200 bladder cytology specimens consisting of four groups with 50 patients each in the following cytologic categories: (1) no evidence of malignancy or dysplasia (controls), (2) PV, (3) urothelial dysplasia (UD), and (4) high grade transitional cell carcinoma (TCC-HG). For each case, two slides with 25-ram filter imprints were stained, one using the Papanicolaou method and the other using the Feulgen staining method. The DNA index (DI), proliferative activity (S+G2M) and degree of hyperploidy (> 5C) were evaluated using an image analysis system. RESULTS: Using the Wilcoxon rank-sum test, statistically significant differences in the DI were found between the PV and UD groups (P = .008) and between the PV and TCC-HG groups (P <.0001). There was no significant difference in the DI between the PV and control groups. The S+G2M fraction for the PV group significantly differed from the control, UD and TCC-HG groups (all P<.0001). Between all four groups, the degrees of hyperploidy were significantly different as well (all P <.0001). CONCLUSION: Cytologically confirmed PV-infected urothelial cells demonstrated a unique DNA content pat- tern with mildly elevated proliferative activity and a significantly dispersed hyperploid DNA content pattern. DNA analysis can help to differentiate PV infection from dysplasia and high grade carcinoma.",
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T1 - Comparative analysis of DNA content in polyomavirus-infected urothelial cells, urothelial dysplasia and high grade transitional cell carcinoma

AU - Wojcik, Eva M.

AU - Miller, M. Craig

AU - Wright, Bradley C.

AU - Veltri, Robert W.

AU - O'Dowd, Gerard J.

PY - 1997

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N2 - OBJECTIVE: To characterize the DNA content pattern in cytologically confirmed polyomavirus (PV)-infected urothelial cells and to compare it with DNA ploidy changes in cytologically diagnosed urothelial dysplasia and high grade transitional cell carcinoma. STUDY DESIGN: We selected 200 bladder cytology specimens consisting of four groups with 50 patients each in the following cytologic categories: (1) no evidence of malignancy or dysplasia (controls), (2) PV, (3) urothelial dysplasia (UD), and (4) high grade transitional cell carcinoma (TCC-HG). For each case, two slides with 25-ram filter imprints were stained, one using the Papanicolaou method and the other using the Feulgen staining method. The DNA index (DI), proliferative activity (S+G2M) and degree of hyperploidy (> 5C) were evaluated using an image analysis system. RESULTS: Using the Wilcoxon rank-sum test, statistically significant differences in the DI were found between the PV and UD groups (P = .008) and between the PV and TCC-HG groups (P <.0001). There was no significant difference in the DI between the PV and control groups. The S+G2M fraction for the PV group significantly differed from the control, UD and TCC-HG groups (all P<.0001). Between all four groups, the degrees of hyperploidy were significantly different as well (all P <.0001). CONCLUSION: Cytologically confirmed PV-infected urothelial cells demonstrated a unique DNA content pat- tern with mildly elevated proliferative activity and a significantly dispersed hyperploid DNA content pattern. DNA analysis can help to differentiate PV infection from dysplasia and high grade carcinoma.

AB - OBJECTIVE: To characterize the DNA content pattern in cytologically confirmed polyomavirus (PV)-infected urothelial cells and to compare it with DNA ploidy changes in cytologically diagnosed urothelial dysplasia and high grade transitional cell carcinoma. STUDY DESIGN: We selected 200 bladder cytology specimens consisting of four groups with 50 patients each in the following cytologic categories: (1) no evidence of malignancy or dysplasia (controls), (2) PV, (3) urothelial dysplasia (UD), and (4) high grade transitional cell carcinoma (TCC-HG). For each case, two slides with 25-ram filter imprints were stained, one using the Papanicolaou method and the other using the Feulgen staining method. The DNA index (DI), proliferative activity (S+G2M) and degree of hyperploidy (> 5C) were evaluated using an image analysis system. RESULTS: Using the Wilcoxon rank-sum test, statistically significant differences in the DI were found between the PV and UD groups (P = .008) and between the PV and TCC-HG groups (P <.0001). There was no significant difference in the DI between the PV and control groups. The S+G2M fraction for the PV group significantly differed from the control, UD and TCC-HG groups (all P<.0001). Between all four groups, the degrees of hyperploidy were significantly different as well (all P <.0001). CONCLUSION: Cytologically confirmed PV-infected urothelial cells demonstrated a unique DNA content pat- tern with mildly elevated proliferative activity and a significantly dispersed hyperploid DNA content pattern. DNA analysis can help to differentiate PV infection from dysplasia and high grade carcinoma.

KW - Bladder neoplasm

KW - Carcinoma

KW - DNA

KW - Ploidies

KW - Polyomavirus

KW - Transitional cell

KW - Urothelium

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