Compact units in proteins

Micheal H. Zehfus, George D Rose

Research output: Contribution to journalArticle

Abstract

An explicit measure of geometric compactness called the coefficient of compactness is introduced. This single value figure of merit identifies those continuous segments of the polypeptide chain having the smallest solvent-accessible surface area for their volume. These segments are the most compact units of the protein, and the larger ones correspond to conventional protein domains. To demonstrate the plausibility of this approach as a method of identifying protein domains, the measure is applied to lysozyme and ribonuclease to discover their constituent compact units. These units are then compared with domains, subdomains, and modules found by other methods. To show the sensitivity of the method, the measure is used to successfully differentiate between native and deliberately misfolded proteins [Novotný, J., Bruccoleri, R., & Karplus, M. (1984) J. Mol. Biol. 177, 787-818]. Methods that utilize only backbone atoms to define domains cannot distinguish between authentic and misfolded molecules because their backbone conformations are virtually superimposable. Compact units identified by this method exhibit a hierarchic organization. Such an organization suggests possible folding pathways that can be tested experimentally.

Original languageEnglish (US)
Pages (from-to)5759-5765
Number of pages7
JournalBiochemistry®
Volume25
Issue number19
StatePublished - 1986
Externally publishedYes

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Proteins
Ribonucleases
Muramidase
Conformations
Atoms
Peptides
Molecules
Protein Domains

ASJC Scopus subject areas

  • Biochemistry

Cite this

Zehfus, M. H., & Rose, G. D. (1986). Compact units in proteins. Biochemistry®, 25(19), 5759-5765.

Compact units in proteins. / Zehfus, Micheal H.; Rose, George D.

In: Biochemistry®, Vol. 25, No. 19, 1986, p. 5759-5765.

Research output: Contribution to journalArticle

Zehfus, MH & Rose, GD 1986, 'Compact units in proteins', Biochemistry®, vol. 25, no. 19, pp. 5759-5765.
Zehfus MH, Rose GD. Compact units in proteins. Biochemistry®. 1986;25(19):5759-5765.
Zehfus, Micheal H. ; Rose, George D. / Compact units in proteins. In: Biochemistry®. 1986 ; Vol. 25, No. 19. pp. 5759-5765.
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