Combined intra-tendinous injection of Platelet Rich Plasma and bevacizumab accelerates and improves healing compared to Platelet Rich Plasma in tendinosis: Comprehensive assessment on a rat model

Benjamin Dallaudiere, Olivier Zurlinden, Anne Perozziello, Lydia Deschamps, Ahmed Larbi, Liliane Louedec, Lionel Pesquer, Yohan Benayoun, Alain Silvestre, Jean Michel Serfaty

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: the aim of our study was to assess the potential of combined intratendinous injection of an anti-angiogenic drug: bevacizumab (AA) and Platelet Rich Plasma (PRP) to treat tendinopathy in a murine model of patellar and Achilles tendinopathy, and to evaluate its local toxicity. Material andMaterlal and method: twenty rats (80 patellar and Achilles tendons) were used for the study. We induced tendinosis (T+) in 80 tendons (patellar=40 and Achilles=40) by injecting under ultrasonography (US) guidance Collagenase 1® (day 0 = D0). Clinical examination was performed at D3, immediately followed by either PRP and AA (AAPRPT+, n=40) or PRP (PRPT+ n=40, control) US-guided intratendinous injection. Follow-up at D6, D18 and D25 using clinical, US and histology, and comparison between the 2 groups were performed. To study AA+PRP toxicity, we looked for necrosis or rupture on the 40 AAPRPT+.Results: all AAPRPT+ showed better joint mobilization compared to PRPT+ at D6 (p=0.03), D18 (p=0.04) and D25 (p=0.02). Similar results were found regarding US and histology, with smaller collagen fiber diameters (D6, p≤0.017, D25, p≤0.015), less disorganization and fewer neovessels (D25, p=0.004) in AAPRPT+ compared to PRPT+. No AA+PRP local toxicity was discovered in histology assessment.Conclusion: our study suggests that combined injection of AA and PRP in tendinosis accelerates and improves tendon’s healing compared PRP used alone, with no local toxicity.

Original languageEnglish (US)
Pages (from-to)351-356
Number of pages6
JournalMuscles, Ligaments and Tendons Journal
Volume4
Issue number3
DOIs
StatePublished - Jul 1 2014
Externally publishedYes

Keywords

  • Anti-angiogenic
  • PRP
  • Rat
  • Tendinosis
  • Tendon
  • US

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Fingerprint Dive into the research topics of 'Combined intra-tendinous injection of Platelet Rich Plasma and bevacizumab accelerates and improves healing compared to Platelet Rich Plasma in tendinosis: Comprehensive assessment on a rat model'. Together they form a unique fingerprint.

Cite this