Combination gene transfer to potentiate tumor regression

T. Ohno, Z. Yang, X. Ling, M. Jaffe, E. G. Nabel, D. Normolle, G. J. Nabel

Research output: Contribution to journalArticle

Abstract

Recent efforts to treat malignancy using gene transfer have met with varying degrees of success. In this paper, we report the results of studies using two recombinant adenoviral vectors to examine the efficacy of combination gene transfer to cause tumor regression in vivo. One of these vectors encodes the murine MHC class 1 gene, H-2Kb (ADV-Kb), which induces an immune response that stimulates tumor regression. The second vector encodes the human p21 cyclin dependent kinase inhibitor (ADV-p21). This gene product arrests cell cycle progression and prevents proliferation of tumor cells. Both vectors were tested in a murine model in vivo for antitumor effect. As previously shown, a significant reduction of tumor size was observed with each vector. Combination treatment, in which both vectors were administered, resulted in a trend toward a reduced tumor growth greater than with either vector alone. In order to characterize the mechanism of tumor regression, cytolytic T lymphocyte (CTL) assays against the allogenic molecule, H-2Kb, were performed. Mice treated with ADV-Kb showed specific CTL activity against the H-2Kb molecule, demonstrating that the immune response against the H-2Kb gene product involved in tumor regression which affects cell cycle progression.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalGene Therapy
Volume4
Issue number4
StatePublished - 1997
Externally publishedYes

Keywords

  • Cancer
  • Gene therapy
  • MHC
  • p21

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Ohno, T., Yang, Z., Ling, X., Jaffe, M., Nabel, E. G., Normolle, D., & Nabel, G. J. (1997). Combination gene transfer to potentiate tumor regression. Gene Therapy, 4(4), 361-366.