Column-switching HPLC for the analysis of plasma in PET imaging studies

John Hilton; Fuji Yokoi; Robert F. Dannals; Hayden T. Ravert; Zsolt Szabo; Dean F. Wong

doi:10.1016/S0969-8051(00)00125-6

Column-switching HPLC for the analysis of plasma in PET imaging studies

John Hilton, Fuji Yokoi, Robert F. Dannals, Hayden T. Ravert, Zsolt Szabo, Dean F. Wong

School of Medicine

Research output: Contribution to journal › Article › peer-review

153 Scopus citations

Abstract

A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [¹¹C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea. (C) 2000 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	627-630
Number of pages	4
Journal	Nuclear Medicine and Biology
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/S0969-8051(00)00125-6
State	Published - 2000

Keywords

Column-switch
HPLC
PET
Plasma

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Cancer Research

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10.1016/S0969-8051(00)00125-6

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title = "Column-switching HPLC for the analysis of plasma in PET imaging studies",

abstract = "A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [11C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea. (C) 2000 Elsevier Science Inc.",

keywords = "Column-switch, HPLC, PET, Plasma",

author = "John Hilton and Fuji Yokoi and Dannals, {Robert F.} and Ravert, {Hayden T.} and Zsolt Szabo and Wong, {Dean F.}",

note = "Funding Information: This work was supported in part by Grants AA11653, AG14400, and DK50183 from the NIH awarded to Dr. Zsolt Szabo, and NIH Grants DA11080, DA09428, MH42821, HD24448, and HD24061 and The Scottish Rite Foundation and NARSAD grants awarded to Dr. Dean F. Wong.",

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doi = "10.1016/S0969-8051(00)00125-6",

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T1 - Column-switching HPLC for the analysis of plasma in PET imaging studies

AU - Hilton, John

AU - Yokoi, Fuji

AU - Dannals, Robert F.

AU - Ravert, Hayden T.

AU - Szabo, Zsolt

AU - Wong, Dean F.

N1 - Funding Information: This work was supported in part by Grants AA11653, AG14400, and DK50183 from the NIH awarded to Dr. Zsolt Szabo, and NIH Grants DA11080, DA09428, MH42821, HD24448, and HD24061 and The Scottish Rite Foundation and NARSAD grants awarded to Dr. Dean F. Wong.

PY - 2000

Y1 - 2000

N2 - A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [11C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea. (C) 2000 Elsevier Science Inc.

AB - A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [11C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea. (C) 2000 Elsevier Science Inc.

KW - Column-switch

KW - HPLC

KW - PET

KW - Plasma

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JF - Nuclear Medicine and Biology

IS - 6

ER -

Column-switching HPLC for the analysis of plasma in PET imaging studies

Abstract

Keywords

ASJC Scopus subject areas

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