Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina. The procedures described include loading the column, assembling the apparatus, separating compounds and collecting fractions, and optimizing chromatography conditions.
|Original language||English (US)|
|Pages (from-to)||Appendix 3E|
|Journal||Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]|
|State||Published - May 2001|
ASJC Scopus subject areas
- Organic Chemistry