Abstract
Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZα (encoding the α-polypeptide fragment of β-galactosidase (βGal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of βGal color selection in appropriate E. coli host strains.
Original language | English (US) |
---|---|
Pages (from-to) | 181-182 |
Number of pages | 2 |
Journal | Gene |
Volume | 166 |
Issue number | 1 |
DOIs | |
State | Published - Dec 1 1995 |
Keywords
- Shuttle plasmid
- color selection
- hygromycin gene
- lacZ gene
- multiple cloning site
- mycobacteria
ASJC Scopus subject areas
- Genetics