Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector

Nathan S. Howard, James E. Gomez, Chiew Ko, William R. Bishai

Research output: Contribution to journalArticlepeer-review

Abstract

Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZα (encoding the α-polypeptide fragment of β-galactosidase (βGal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of βGal color selection in appropriate E. coli host strains.

Original languageEnglish (US)
Pages (from-to)181-182
Number of pages2
JournalGene
Volume166
Issue number1
DOIs
StatePublished - Dec 1 1995

Keywords

  • Shuttle plasmid
  • color selection
  • hygromycin gene
  • lacZ gene
  • multiple cloning site
  • mycobacteria

ASJC Scopus subject areas

  • Genetics

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