Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow

Monica M. Burdick, J. Michael McCaffery, Young S. Kim, Bruce S. Bochner, K Konstantopoulos

Research output: Contribution to journalArticle

Abstract

This study was undertaken to investigate the molecular constituents mediating LS174T colon adenocarcinoma cell adhesion to 4-h TNF-α-stimulated human umbilical vein endothelial cells (HUVECs) under flow. At 1 dyn/cm2, ∼57% of cells rolled and then became firmly adherent, whereas others continuously rolled on endothelium. Initial cell binding was primarily mediated by endothelial E-selectin. By using neuraminidase, glycolipid biosynthesis inhibitor d,l-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol·HCl, trypsin, and flow cytometry, LS174T cells were shown to express sialyl Lewisx(sLex)- and di-sLex-decorated, but not sLea-decorated, glycolipid and glycoprotein ligands for E-selectin. The cells preferentially employed sialylated glycoproteins over glycolipids in adhesion as measured by conversion of rolling to firm adhesion, resistance to detachment by increased shear stress, and rolling velocity. However, a nonsialylated E-selectin counterreceptor also exists. Furthermore, LS174T α2, α6, and β1, integrins support a minor pathway in adhesion to HUVECs. Finally, tumor cell attachment specifically increases HUVEC endocytosis of E-selectin. Altogether, the data indicate the complexity of carcinoma cell-endothelium adhesion via sialylated glycoconjugates, integrins, and their respective counterreceptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number4 53-4
StatePublished - Apr 1 2003

Fingerprint

E-Selectin
Glycolipids
Endothelial cells
Human Umbilical Vein Endothelial Cells
Integrins
Glycoproteins
Colon
Adhesion
Cells
Carcinoma
Cell Adhesion
Endothelium
Glycoconjugates
Flow cytometry
Biosynthesis
Cell adhesion
Neuraminidase
Trypsin
Shear stress
Tumors

Keywords

  • E-selectin
  • Glycolipid
  • Shear stress
  • Sialyl Lewis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. / Burdick, Monica M.; Michael McCaffery, J.; Kim, Young S.; Bochner, Bruce S.; Konstantopoulos, K.

In: American Journal of Physiology - Cell Physiology, Vol. 284, No. 4 53-4, 01.04.2003.

Research output: Contribution to journalArticle

Burdick, Monica M. ; Michael McCaffery, J. ; Kim, Young S. ; Bochner, Bruce S. ; Konstantopoulos, K. / Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. In: American Journal of Physiology - Cell Physiology. 2003 ; Vol. 284, No. 4 53-4.
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