Collective cancer cell invasion induced by coordinated contractile stresses

Angela M Jimenez Valencia, Pei Hsun Wu, Osman N. Yogurtcu, Pranay Rao, Josh DiGiacomo, Inês Godet, Lijuan He, Meng Horng Lee, Daniele Gilkes, Sean X. Sun, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

The physical underpinnings of fibrosarcoma cell dissemination from a tumor in a surrounding collagen-rich matrix are poorly understood. Here we show that a tumor spheroid embedded in a 3D collagen matrix exerts large contractile forces on the matrix before invasion. Cell invasion is accompanied by complex spatially and temporally dependent patterns of cell migration within and at the surface of the spheroids that are fundamentally different from migratory patterns of individual fibrosarcoma cells homogeneously distributed in the same type of matrix. Cells display a continuous transition from a round morphology at the spheroid core, to highly aligned elongated morphology at the spheroid periphery, which depends on both β1- integrin-based cell-matrix adhesion and myosin II/ROCK-based cell contractility. This isotropic-to-anisotropic transition corresponds to a shift in migration, from a slow and unpolarized movement at the core, to a fast, polarized and persistent one at the periphery. Our results also show that the ensuing collective invasion of fibrosarcoma cells is induced by anisotropic contractile stresses exerted on the surrounding matrix.

Original languageEnglish (US)
Pages (from-to)43438-43451
Number of pages14
JournalOncotarget
Volume6
Issue number41
DOIs
StatePublished - 2015

Fingerprint

Fibrosarcoma
Neoplasms
Collagen
Cell-Matrix Junctions
Myosin Type II
Integrins
Cell Movement

Keywords

  • 3D invasion model
  • Fibrosarcoma
  • Spatio-temporal invasion

ASJC Scopus subject areas

  • Oncology

Cite this

Valencia, A. M. J., Wu, P. H., Yogurtcu, O. N., Rao, P., DiGiacomo, J., Godet, I., ... Wirtz, D. (2015). Collective cancer cell invasion induced by coordinated contractile stresses. Oncotarget, 6(41), 43438-43451. https://doi.org/10.18632/oncotarget.5874

Collective cancer cell invasion induced by coordinated contractile stresses. / Valencia, Angela M Jimenez; Wu, Pei Hsun; Yogurtcu, Osman N.; Rao, Pranay; DiGiacomo, Josh; Godet, Inês; He, Lijuan; Lee, Meng Horng; Gilkes, Daniele; Sun, Sean X.; Wirtz, Denis.

In: Oncotarget, Vol. 6, No. 41, 2015, p. 43438-43451.

Research output: Contribution to journalArticle

Valencia, AMJ, Wu, PH, Yogurtcu, ON, Rao, P, DiGiacomo, J, Godet, I, He, L, Lee, MH, Gilkes, D, Sun, SX & Wirtz, D 2015, 'Collective cancer cell invasion induced by coordinated contractile stresses', Oncotarget, vol. 6, no. 41, pp. 43438-43451. https://doi.org/10.18632/oncotarget.5874
Valencia AMJ, Wu PH, Yogurtcu ON, Rao P, DiGiacomo J, Godet I et al. Collective cancer cell invasion induced by coordinated contractile stresses. Oncotarget. 2015;6(41):43438-43451. https://doi.org/10.18632/oncotarget.5874
Valencia, Angela M Jimenez ; Wu, Pei Hsun ; Yogurtcu, Osman N. ; Rao, Pranay ; DiGiacomo, Josh ; Godet, Inês ; He, Lijuan ; Lee, Meng Horng ; Gilkes, Daniele ; Sun, Sean X. ; Wirtz, Denis. / Collective cancer cell invasion induced by coordinated contractile stresses. In: Oncotarget. 2015 ; Vol. 6, No. 41. pp. 43438-43451.
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